In vivo imaging of transplanted hematopoietic stem and progenitor cells in mouse calvarium bone marrow.
In vivo imaging of transplanted hematopoietic stem and progenitor cells in mouse calvarium bone marrow.
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DOI：
10.1038/nprot.2010.168
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发表时间：
2011-01
期刊：
影响因子：
14.8
通讯作者：
Scadden, David T.
中科院分区：
文献类型：
--
作者：
Lo Celso, Cristina;Lin, Charles P.;Scadden, David T.
开发了移植的造血干细胞和祖细胞（HSPCs）的体内成像以研究HSPCs与其在骨髓中的微环境组分之间的关系。特别是，它允许直接观察造血细胞在移植后最初几天内的行为，此时正在发生归巢和早期植入的关键事件。通过直接对活体动物中的这些事件进行成像，该方法允许详细评估先前通过细胞计数的粗略评估（归巢）或延长时间后（植入）评估的功能。该方案提供了一种新的手段，调查的作用细胞内在和细胞外在的造血分子调节剂在移植的早期阶段，它是第一个允许在三维和真实的时间内骨髓内的细胞-细胞相互作用的研究。在本文中，我们描述了如何分离，标记和注射HSPCs，以及如何进行颅骨活体显微镜检查和分析所得的图像。一个典型的实验可以在大约1周内完成并分析。
In vivo imaging of transplanted hematopoietic stem and progenitor cells (HSPCs) was developed to investigate the relationship between HSPCs and components of their microenvironment in the bone marrow. In particular, it allows a direct observation of the behavior of hematopoietic cells during the first few days after transplantation, when the critical events in homing and early engraftment are occurring. By directly imaging these events in living animals, this method permits a detailed assessment of functions previously evaluated by crude assessments of cell counts (homing) or after prolonged periods (engraftment). This protocol offers a new means of investigating the role of cell-intrinsic and cell-extrinsic molecular regulators of hematopoiesis during the early stages of transplantation, and it is the first to allow the study of cell-cell interactions within the bone marrow in three dimensions and in real time. In this paper, we describe how to isolate, label and inject HSPCs, as well as how to perform calvarium intravital microscopy and analyze the resulting images. A typical experiment can be performed and analyzed in ~1 week.
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影响因子：
64.8
作者：
Adams, Gregor B.;Alley, Ian R.;Chung, Ung-il;Chabner, Karissa T.;Jeanson, Nathaniel T.;Lo Celso, Cristina;Marsters, Emily S.;Chen, Min;Weinstein, Lee S.;Lin, Charles P.;Kronenberg, Henry M.;Scadden, David T.
通讯作者：
Scadden, David T.
影响因子：
64.5
作者：
Kiel, MJ;Yilmaz, ÖH;Morrison, SJ
通讯作者：
Morrison, SJ
影响因子：
20.3
作者：
Lewandowski, Daniel;Barroca, Vilma;Romeo, Paul-Henri
通讯作者：
Romeo, Paul-Henri
DOI：
10.1109/jstqe.2007.912751
发表时间：
2008-01-01
影响因子：
4.9
作者：
Veilleux, Israel;Spencer, Joel A.;Lin, Charles P.
通讯作者：
Lin, Charles P.
影响因子：
64.8
作者：
Chan, Charles K. F.;Chen, Ching-Cheng;Luppen, Cynthia A.;Kim, Jae-Beom;DeBoer, Anthony T.;Wei, Kevin;Helms, Jill A.;Kuo, Calvin J.;Kraft, Daniel L.;Weissman, Irving L.
通讯作者：
Weissman, Irving L.