CD44 protein levels and its biological activity are regulated in Balb/c 3T3 fibroblasts by serum factors and by transformation with the ras but not with the sis Oncogene
CD44 protein levels and its biological activity are regulated in Balb/c 3T3 fibroblasts by serum factors and by transformation with the ras but not with the sis Oncogene
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Balb/c 3T3 成纤维细胞中的 CD44 蛋白水平及其生物活性受血清因子和 ras 转化调节，但不受 sis Oncogene 调节
DOI：
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发表时间：
1996
影响因子：
5.6
通讯作者：
L. Culp
中科院分区：
文献类型：
--
作者：
P. Kogerman;M. Sy;L. Culp
在经ras或sis癌基因转化的Balb/c 3 T3细胞及其衍生物中研究了CD 44 s（标准亚型）水平和透明质酸结合活性，作为培养基中血清浓度的函数。3 T3细胞含有低水平的CD 44，并且当在含有0.5%或10%血清的培养基中生长时不结合透明质酸。然而，在5%血清中，细胞具有高得多的CD 44水平，并且能够结合透明质酸。在先前维持在低血清中后，用5%或10%血清重新刺激的3 T3细胞中CD 44水平也增加。在5%血清再刺激的细胞中，高水平的CD 44可维持至少72小时，而在10%血清再刺激的细胞中，CD 44水平的增加在48小时后恢复。（但不与sis）癌基因使CD 44水平对血清调节不敏感：在所有血清浓度和所有检测时间点，ras转化细胞均含有高水平的CD 44和结合透明质酸。在这些调节变化中，Sis转化细胞表现得像3 T3细胞。当补充0.5%血清时，血小板衍生生长因子（PDGF）模拟血清对3 T3细胞中CD 44水平和透明质酸结合能力的影响，血清的CD 44上调活性通过与抗PDGF抗体孵育而被中和。这些数据表明，血清因子，特别是PDGF，介导的Balb/c 3 T3细胞中的CD 44水平的调节和ras的3 T3细胞的转化使得CD 44表达对血清的体外调节作用不敏感。这些结果与这些细胞在体内的转移能力相关。© 1996 Wiley利斯公司
CD44s (standard isoform) levels and hyaluronan‐binding activity were investigated in Balb/c 3T3 cells and their derivatives transformed with ras or sis oncogenes as a function of serum concentration in the medium. 3T3 cells contained low levels of CD44 and did not bind hyaluronan when grown in medium containing 0.5 or 10% serum. In 5% serum, however, the cells had much higher levels of CD44 and were able to bind hyaluronan. CD44 levels also increased in 3T3 cells restimulated with either 5 or 10% serum after prior maintenance in low serum. In cells restimulated with 5% serum, high levels of CD44 were sustained for at least 72 hr. In cells restimulated with 10% serum, however, the increase in CD44 levels reverted by 48 hr. Transformation of 3T3 cells with ras (but not with sis) oncogene rendered CD44 levels insensitive to serum modulation: ras‐transformed cells contained high levels of CD44 and bound hyaluronan at all serum concentrations and at all time points tested. Sis‐transformed cells behaved like 3T3 cells in these modulatory changes. Platelet‐derived growth factor (PDGF), when supplementing 0.5% serum, mimicked the effects of serum on the levels and hyaluronan‐binding capacity of CD44 in 3T3 cells and the CD44‐upregulating activity of serum was neutralized by incubation with anti‐PDGF antibodies. These data demonstrate that serum factors, specifically PDGF, mediate regulation of CD44 levels in Balb/c 3T3 cells and that transformation of 3T3 cells by ras renders CD44 expression insensitive to the modulating effects of serum in vitro. These results correlate with the metastatic capacity of these cells in vivo. © 1996 Wiley‐Liss, Inc.
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细菌 lacZ 基因作为检测肿瘤进展过程中微转移形成的高度敏感标记。
影响因子：
11.2
作者：
Lin,WC;Pretlow,TP;Pretlow2nd,TG;Culp,LA
通讯作者：
Culp,LA
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DOI：
--
发表时间：
1987
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
Keating,MT;Williams,LT
通讯作者：
Williams,LT
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DOI：
--
发表时间：
1991
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
Drozdoff,V;Pledger,WJ
通讯作者：
Pledger,WJ
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DOI：
10.1073/pnas.84.15.5143
发表时间：
1987
影响因子：
11.1
作者：
Radinsky,R;Kraemer,PM;Raines,MA;Kung,HJ;Culp,LA
通讯作者：
Culp,LA
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影响因子：
11.2
作者：
Radinsky,R;Kraemer,PM;Proffitt,MR;Culp,LA
通讯作者：
Culp,LA