Differentiation of dendritic cells in cultures of rat bone marrow cells.
Differentiation of dendritic cells in cultures of rat bone marrow cells.
复制标题
DOI：
10.1084/jem.163.4.872
复制
发表时间：
1986-04-01
期刊：
影响因子：
--
通讯作者：
Berkowitz MR
中科院分区：
文献类型：
--
作者：
Bowers WE;Berkowitz MR
虽然树突状细胞（DC）来源于骨髓，但在新鲜骨髓细胞（BMC）中未观察到树突状细胞。同样，辅助活性在一个敏感的试验中几乎无法测量到DC的这种有效功能。然而，当BMC培养5 d时，DC和附属活性都得到了发展。基于培养前的分离，几乎所有的附属活性都可以归因于低密度（LD）馏分中恢复的总BMC的5%。lcd -DC前体在许多重要方面与成熟的DC不同。当培养非贴壁细胞时，通过筛洗从LD部分去除Ia+细胞并没有减少DC的产生。因此，与骨髓培养中产生的强Ia+ DC相比，DC来源于的细胞不表达或最低限度地表达Ia抗原。培养前对LD细胞进行照射可阻止DC细胞的发育。当每隔一天延迟照射时，DC的数量会逐渐增加，直至第五天。这些发现，连同初步的放射自显像数据，表明细胞分裂已经发生，而DC则不分裂。我们得出结论，骨髓源性DC是由LD、Ia-前体分化而成。
Although dendritic cells (DC) originate from bone marrow, they were not observed in fresh preparations of bone marrow cells (BMC). Likewise, accessory activity was barely measurable in a sensitive assay for this potent function of DC. However, both DC and accessory activity developed when BMC were cultured for 5 d. Based on fractionation before culture, nearly all of the accessory activity could be attributed to only 5% of the total BMC recovered in a low-density (LD) fraction. The LD-DC precursors differed from mature DC in a number of important respects. Removal of Ia+ cells from the LD fraction by panning did not decrease the production of DC when the nonadherent cells were cultured. Thus, the cell from which the DC is derived does not express or minimally expresses Ia antigens, in contrast to the strongly Ia+ DC that is produced in bone marrow cultures. Irradiation of LD cells before culture prevented the development of DC. When irradiation was delayed by daily intervals, progressive increases in the number of DC resulted, up to the fifth day. These findings, together with preliminary autoradiographic data, indicate that cell division has occurred, in contrast to the DC, which does not divide. We conclude that bone marrow-derived DC arise in culture from the division of LD, Ia- precursors.