Evaluation of lateral-flow clostridium botulinum neurotoxin detection kits for food analysis
Evaluation of lateral-flow clostridium botulinum neurotoxin detection kits for food analysis
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DOI：
10.1128/aem.71.7.3935-3941.2005
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发表时间：
2005-07-01
影响因子：
4.4
通讯作者：
Whiting, RC
中科院分区：
文献类型：
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作者：
Sharma, SK;Eblen, BS;Whiting, RC
两个体外侧向如何检测肉毒梭菌神经毒素（BoNTs）在食品分类的适用性和灵敏度进行了评估。开发了各种液体、固体和高脂肪含量食品的毒素提取和制备方法。侧流测定法，一种由海军医学研究中心（银Spring，MD）开发，另一种由Allexeter Technologies（盖瑟斯堡，MD）开发，基于BoNT A、B和E型的免疫检测。这些分析被认为是快速和易于执行的实验室设备或技能的最低要求。它们可以容易地检测10 ng/ml的A和B型BoNT和20 ng/ml的E型BoNT。与其他体外检测方法相比，这些检测方法的灵敏度较低，并且对结果的评估是严格定性的。然而，发现该测定法使用简单，并且需要最少的培训。该检测方法成功地检测了各种食品中的A、B和E型BoNT，表明其作为初步筛选系统的潜在用途，用于在C型BoNT水平升高的情况下对食品样品进行分类。肉毒杆菌污染事件。
The suitability and sensitivity of two in vitro lateral-How assays for detecting Clostridium botulinum neurotoxins (BoNTs) in an assortment of foods were evaluated. Toxin extraction and preparation methods for various liquid, solid, and high-fat-con tent foods were developed. The lateral-flow assays, one developed by the Naval Medical Research Center (Silver Spring, MD) and the other by Allexeter Technologies (Gaithersburg, MD), are based on the immunodetection of BoNT types A, B, and E. The assays were found to be rapid and easy to perform with minimum requirements for laboratory equipment or skills. They can readily detect 10 ng/ml of BoNT types A and B and 20 ng/ml of BoNT type E. Compared to other in vitro detection methods, these assays are less sensitive, and the assessment of a result is strictly qualitative. However, the assay was found to be simple to use and to require minimal training. The assays successfully detected BoNT types A, B, and E in a wide variety of foods, suggesting their potential usefulness as a preliminary screening system for triaging food samples with elevated BoNT levels in the event of a C. botulinum contamination event.