Overexpression of violaxanthin de-epoxidase gene alleviates photoinhibition of PSII and PSI in tomato during high light and chilling stress
Overexpression of violaxanthin de-epoxidase gene alleviates photoinhibition of PSII and PSI in tomato during high light and chilling stress
复制标题
紫黄素脱环氧化酶基因的过表达减轻了番茄高光和低温胁迫下 PSII 和 PSI 的光抑制
DOI：
10.1016/j.jplph.2009.08.009
复制
发表时间：
2010-01-01
影响因子：
4.3
通讯作者：
Meng, Qing-Wei
中科院分区：
文献类型：
--
作者：
Han, Han;Gao, Shan;Meng, Qing-Wei
克隆了番茄紫黄素脱环氧酶基因(LeVDE)。推导的氨基酸序列与其他植物紫黄质脱环氧酶有很高的同源性。RNA凝胶印迹分析表明，野生型(WT)中LeVDE的mRNA积累受昼夜节律和温度的调节。RNA和蛋白质凝胶印迹分析证实，正义的LeVDE基因已转入番茄基因组，并在35S-CaMV控制下过表达。在4℃强光和低温胁迫下，转基因植株的(A+Z)/(V+A+Z)比值和非光化学猝灭(NPQ)值均高于对照植株。在强光胁迫下，WT的净光合速率(Pn)显著低于转基因品系。在最适条件下，转基因植株的PSII初级光化学最大量子效率(Fv/Fm)下降较慢，恢复较快。在低温胁迫下，转基因植株的可氧化性P700高于对照。这些结果表明，在强光和弱光胁迫下，过表达LeVDE增强了番茄叶黄素循环的功能，缓解了PSII和PSI的光抑制。(C)2009年爱思唯尔股份有限公司。版权所有。
A tomato (Lycopersicon esculentum) violaxanthin de-epoxidase gene (LeVDE) was isolated. The deduced amino acid sequence of LeVDE showed high identities with violaxanthin de-epoxidase in other plant species. RNA gel blot analysis showed that the mRNA accumulation of LeVDE in the wild-type (WT) was regulated by diurnal rhythm and temperature. RNA and protein gel blot analyses confirmed that the sense LeVDE was transferred into the tomato genome and overexpressed under the control of 35S-CaMV. The ratio of (A+Z)/(V+A+Z) and the values of non-photochemical quenching (NPQ) were higher in transgenic plants than those in WT under high light and chilling stress (4 degrees C). The net photosynthetic rate (Pn) decreased markedly in WT compared to transgenic lines under high light stress. The maximum quantum yield of primary photochemistry of PSII (Fv/Fm) in transgenic plants decreased more slowly during stresses and recovered faster than that in WT under optimal conditions. The oxidizable P700 in transgenic plants was higher than that in WT under chilling stress. These results suggest that overexpression of LeVDE increased the function of the xanthophyll cycle and alleviated photoinhibition of PSII and PSI in tomato during high light and chilling stress with low irradiance. (C) 2009 Elsevier GmbH. All rights reserved.