Degradation of amyloid _ peptide by neprilysin expressed from Borna disease virus vector.
Degradation of amyloid _ peptide by neprilysin expressed from Borna disease virus vector.
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博尔纳病病毒载体表达的脑啡肽酶对淀粉样蛋白_肽的降解。
DOI：
10.1111/1348-0421.12602
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发表时间：
2018
期刊：
影响因子：
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通讯作者：
Tomonaga K.
中科院分区：
文献类型：
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作者：
Sakai M;Ueda S;Daito T;Asada-Utsugi M;Komatsu Y;Kinoshita A;Maki T;Kuzuya A;Takahashi R;Makino A;Tomonaga K.
β淀粉样蛋白（a β40和a β42）在大脑中的积累是阿尔茨海默病（AD）的一个特征。由于neprilysin （NEP）是一种主要的a β降解酶，因此在大脑中递送NEP是一种很有前途的AD基因治疗方法。博纳病病毒（BoDV）载体使中枢神经系统外源基因的长期转导成为可能。在此，我们对经BoDV载体转导的NEP的蛋白水解能力进行了评价，发现Aβ40和Aβ42的数量显著减少，这表明由BoDV载体表达的NEP具有降解Aβ的功能。
Accumulation of amyloid β (Aβ40 and Aβ42) in the brain is a characteristic of Alzheimer disease (AD). Because neprilysin (NEP) is a major Aβ‐degrading enzyme,NEPdelivery in the brain is a promising gene therapy for AD. Borna disease virus (BoDV) vector enables long‐term transduction of foreign genes in the central nerve system. Here, the proteolytic ability of NEP transduced by the BoDV vector was evaluated and it was found that the amounts of Aβ40 and Aβ42 decreased significantly, suggesting that NEP expressed from the BoDV vector is functional in that it degrades Aβ.