Biological reaction control using topography regulation of nanostructured titanium
Biological reaction control using topography regulation of nanostructured titanium
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DOI：
10.1038/s41598-020-59395-4
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发表时间：
2020-02-12
影响因子：
4.6
通讯作者：
Mizuno, Jun
中科院分区：
文献类型：
--
作者：
Shiozawa, Mayuko;Takeuchi, Haruka;Mizuno, Jun
种植体的微纳米表面形貌对周围细胞的生长有影响。在本研究中，在钛基上制备了具有纳米级表面粗糙度的标准化和可控周期纳米图案，并研究了它们对骨髓基质细胞的影响。细胞增殖实验表明，表面粗糙度为1.7 nm的裸底物比表面粗糙度为0.6 nm的裸底物亲水性低，但增殖能力强。此外，使用后一种底物，可以观察到宽度为100 nm，高度为50或100 nm的线条和凹槽图案的定向细胞生长，而高度为10或25 nm的则没有。在光滑的衬底上，延时显微镜分析显示，在100 nm高度的线和凹槽图案上，80%以上的骨髓细胞沿着线生长和分裂。由于纳米颗粒结构控制细胞增殖速率，纳米线槽结构（50-100 nm）控制细胞迁移、分裂和生长方向，这些标准化的纳米钛结构可以用来阐明表面形貌调节组织对生物材料反应的机制。
The micro- and nanosize surface topography of dental implants has been shown to affect the growth of surrounding cells. In this study, standardized and controlled periodic nanopatterns were fabricated with nanosized surface roughness on titanium substrates, and their influence on bone marrow stromal cells investigated. Cell proliferation assays revealed that the bare substrate with a 1.7 nm surface roughness has lower hydrophilicity but higher proliferation ability than that with a 0.6 nm surface roughness. Further, with the latter substrate, directional cell growth was observed for line and groove patterns with a width of 100 nm and a height of 50 or 100 nm, but not for those with a height of 10 or 25 nm. With the smooth substrate, time-lapse microscopic analyses showed that more than 80% of the bone marrow cells on the line and groove pattern with a height of 100 nm grew and divided along the lines. As the nanosized grain structure controls the cell proliferation rate and the nanosized line and groove structure (50-100 nm) controls cell migration, division, and growth orientation, these standardized nanosized titanium structures can be used to elucidate the mechanisms by which surface topography regulates tissue responses to biomaterials.