Molecular characterization of angiogenic properties of human oral squamous cell carcinoma cells
Molecular characterization of angiogenic properties of human oral squamous cell carcinoma cells
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DOI：
10.1016/s1368-8375(02)00004-0
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发表时间：
2002-10-01
期刊：
影响因子：
4.8
通讯作者：
Wu, LW
中科院分区：
文献类型：
--
作者：
Chen, YJ;Jin, YT;Wu, LW
关于口腔癌细胞血管生成特性的特异性及其可能的机制知之甚少。对人脐静脉内皮细胞 (HUVEC) 增殖和迁移的刺激作用表征了口腔癌细胞的血管生成特性，但不是正常口腔角质形成细胞 (NOK) 的血管生成特性。 ELISA 发现测试的口腔癌细胞和 NOK 中均存在血管内皮生长因子 (VEGF)。通过中和 VEGF 抗体来减弱促血管生成作用表明 VEGF 在口腔癌细胞中获得血管生成表型中发挥着关键作用。 p53 和鼠双突变体 2 (Mdm2) 的蛋白质印迹以及 p53 DNA 测序分析表明，无论其病因如何，所有测试的口腔癌细胞中都发生了 Mdm2 突变或过度表达导致的 p53 功能丧失。总之，口腔癌细胞的血管生成特性除了VEGF和p53的功能状态之外，还受到许多因素的介导。 (C) 2002 Elsevier Science Ltd. 保留所有权利。
Little is known about the specificity of angiogenic properties of oral cancer cells and the possible mechanisms. Stimulatory effects on proliferation and migration of human umbilical vein endothelial cells (HUVEC) characterized the angiogenic properties of oral cancer cells but not normal oral keratinocytes (NOK). ELISA found the presence of vascular endothelial growth factors (VEGF) both in the tested oral cancer cells and NOK. Attenuation of the proangiogenic effects by neutralizing VEGF antibodies suggests VEGF play a key role in the acquisition of the angiogenic phenotype in oral cancer cells. Western blotting of p53 and murine double mutant 2 (Mdm2) together with p53 DNA sequencing analysis indicate that p53 function loss by mutation or overexpression of Mdm2 occurred in all tested oral cancer cells regardless of their etiology. In summary, the angiogenic property of oral cancer cells is mediated by many factors in addition to VEGF and the functional status of p53. (C) 2002 Elsevier Science Ltd. All rights reserved.