Molecular characterisation of genital human papillomavirus among women in Southwestern, Nigeria
Molecular characterisation of genital human papillomavirus among women in Southwestern, Nigeria
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DOI：
10.1371/journal.pone.0224748
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发表时间：
2019-11-04
期刊：
影响因子：
3.7
通讯作者：
Odaibo, Georgina N.
中科院分区：
文献类型：
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作者：
Nejo, Yewande T.;Olaleye, David O.;Odaibo, Georgina N.
研究背景高危型生殖器人乳头瘤病毒（HPV）尤其是16型和18型的持续感染与宫颈癌有关。然而，HPV类型的分布在不同的地理区域差异很大，可用的疫苗仅针对少数类型。这项研究的目的是确定在西南部尼日利亚的HPV类型循环，从而提供必要的信息，有效控制的virus.MethodsEndocervical拭子样本收集从295个同意参加常规宫颈癌筛查，性病诊所和社区为基础的推广计划的妇女。从样本中提取病毒DNA，并使用GP-E6/E7引物通过PCR扩增HPV DNA的共有区。型特异性巢式多重PCR和桑格测序被用来分型的HPV分离株。ResultsIn这项研究中，51（17.3%）个人是阳性的HPV DNA使用共识引物，目标E6/E7基因，但只有48（16.3%）进行基因分型。共检测到15种HPV类型（HPV-6、16、18、31、33、35、42、43、44、52、58、66、74、81、86），其中HPV-31最多（32.8%），其次是HPV-35（17.2%）和HPV-16（15.5%）。还检测到两种罕见的HPV类型; 74和86。HPV-74分离株在E7基因处有三个核苷酸（CCT）插入，翻译成氨基酸脯氨酸。最高的核苷酸替换（n = 32）被发现在HPV-44基因型。在阳性个体中，20.8%有双重感染，86.2%有高危型HPV types.ConclusionsMultiple人乳头瘤病毒类型共循环的研究。大多数人乳头瘤病毒是高危型，其中31型是最主要的。虽然首次检测到的带有脯氨酸插入的HPV-74的含义尚不清楚，但它可能对病毒的转化潜力有影响。多价HPV疫苗将更有效地控制尼日利亚的感染。
BackgroundPersistent infections with high-risk genital Human papillomavirus (HPV) especially types 16 and 18, are associated with cervical cancer. However, distribution of HPV types varies greatly across geographical regions and the available vaccines target only few types. This study was designed to determine the HPV types circulating in Southwestern Nigeria, thereby providing necessary information for effective control of the virus.MethodsEndocervical swab samples were collected from a total of 295 consenting women attending routine cervical cancer screening, STI clinics and community-based outreach programme. Viral DNA was extracted from the samples and the consensus region of the HPV DNA was amplified by PCR using GP-E6/E7 primers. Type-specific nested multiplex PCR and Sanger sequencing were used to genotype the HPV isolates.ResultsIn this study, 51 (17.3%) individuals were positive for HPV DNA using consensus primers that target the E6/E7 genes but only 48 (16.3%) were genotyped. A total of 15 HPV types (HPV-6, 16, 18, 31, 33, 35, 42, 43, 44, 52, 58, 66, 74, 81, 86) were detected, with HPV-31 being the most predominant (32.8%), followed by HPV-35 (17.2%) and HPV-16 (15.5%). Two rare HPV types; 74 and 86 were also detected. The HPV-74 isolate had three nucleotide (CCT) insertions at E7 gene that translated into amino acid proline. Highest nucleotide substitutions (n = 32) were found in HPV-44 genotype. Among positive individuals, 20.8% had dual infections and 86.2% had High-risk HPV types.ConclusionsMultiple Human papillomavirus types co-circulated in the study. Most of the circulating Human papillomavirus are high-risk type with type 31 being the most predominant. Although the implication of HPV-74 with proline insertion detected for the first time is unknown, it may have effect on the transformation potential of the virus. Polyvalent HPV vaccine will be more effective for the infection control in Nigeria.