Regeneration of plants using nutrient mist culture
Regeneration of plants using nutrient mist culture
复制标题
利用营养雾培养进行植物再生
DOI：
10.1007/bf02623612
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发表时间：
1988
期刊：
影响因子：
--
通讯作者：
K. Giles
中科院分区：
文献类型：
--
作者：
P. Weathers;K. Giles
摘要营养雾用于在新型生物反应器中进行植物组织的体外培养，其中组织在无菌室内的生物惰性筛网上生长，允许多余的培养基从组织中排出。测试的植物包括胡萝卜、番茄、榕树、金鸡纳和芸苔属。后 4 个属在生物反应器内完全再生。组织接种物包括愈伤组织、花药和芽分生组织。所有植物在营养雾中的生长至少与在琼脂中一样好，并且总是产生更多质量更高的芽，并且通常比琼脂培养更快。成本分析估计表明，使用营养雾培养可节省高达 65% 的生产成本（劳动力和材料）。营养雾培养显着改善了植物的微繁殖。
SummaryA nutrient mist was used forin vitro culture of plant tissue in a novel bioreactor, wherein the tissues were grown on a biologically inert screen within a sterile chamber which allows excess media to drain away from the tissue. Plants tested includedDaucus, Lycopersicon, Ficus, Cinchona, andBrassica. The latter 4 genera were fully regenerated within the bioreactor. Tissue inocula included callus, anthers, and shoot meristems. All plants grew at least as well in nutrient mists as in agar and always produced a greater quantity of shoots of a higher quality and often faster than agar cultures. Cost analysis estimates showed up to a 65% savings in production costs (labor and materials) could be realized using nutrient mist culture. Nutrient mist culture offers significant improvements in the micropropagation of plants.