Enhanced synthesis of leukotriene B4 by colonic mucosa in inflammatory bowel disease.
Enhanced synthesis of leukotriene B4 by colonic mucosa in inflammatory bowel disease.
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DOI：
10.1016/s0016-5085(84)80015-3
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发表时间：
1984-03
期刊：
影响因子：
29.4
通讯作者：
P. Sharon;W. Stenson
中科院分区：
文献类型：
--
作者：
P. Sharon;W. Stenson
白三烯 B4 是一种花生四烯酸代谢物，是一种有效的趋化剂，被认为是重要的炎症介质。为了研究该化合物作为炎症性肠病炎症介质的作用，将花生四烯酸与来自炎症性肠病患者和正常受试者的离子载体和结肠粘膜一起孵育。炎症性肠病患者的粘膜将 2.17% 的外源性花生四烯酸转化为白三烯 B4；正常受试者的粘膜转化为0.37%。柳氮磺吡啶阻断白三烯的产生。为了确定炎症性肠粘膜是否含有内源性白三烯 B4，通过高压液相色谱分析脂质提取物。炎症性肠病患者的粘膜每克含有 254 纳克白三烯 B4，而正常受试者的粘膜每克含有 < 5 纳克白三烯 B4。炎症性肠病患者的结肠粘膜中存在大量白三烯 B4，加上白三烯 B4 的已知生物学作用，表明它可能是炎症性肠病炎症的重要介质。
Leukotriene B4, an arachidonic acid metabolite, is a potent chemotactic agent, and is thought to be an important mediator of inflammation. To investigate the role of this compound as a mediator of inflammation in inflammatory bowel disease, arachidonic acid was incubated with ionophore and colonic mucosa from patients with inflammatory bowel disease and from normal subjects. Mucosa from patients with inflammatory bowel disease converted 2.17% of exogenous arachidonate to leukotriene B4; mucosa from normal subjects converted 0.37%. The production of leukotriene was blocked by sulfasalazine. To determine if inflammatory bowel mucosa contained endogenous leukotriene B4, lipid extracts were analyzed by high pressure liquid chromatography. Mucosa from patients with inflammatory bowel disease contained 254 ng of leukotriene B4per gram and mucosa from normal subjects contained < 5 ng of leukotriene B4per gram. The presence of significant amounts of leukotriene B4in colonic mucosa in patients with inflammatory bowel disease, combined with the known biologic effects of leukotriene B4, suggests that it may be an important mediator of inflammation in inflammatory bowel disease.