Quantitative Colorimetric Analysis of Some Inorganic Salts Using Digital Photography
Quantitative Colorimetric Analysis of Some Inorganic Salts Using Digital Photography
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DOI：
10.1080/00032719.2010.520394
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发表时间：
2011-01-01
影响因子：
2
通讯作者：
Martinez Calatayud, J.
中科院分区：
文献类型：
--
作者：
Lahuerta Zamora, L.;Mellado Romero, A. M.;Martinez Calatayud, J.
本文报道了一种定量分析在可见光谱区吸收的溶解化学物质的分析成像方法。该方法使用目标物质的一系列标准品溶液的数字图像，并使用适当的软件对其进行处理，以根据颜色强度为每个标准品分配数值。这样的值与分析物浓度成正比，并允许构建校准图。由此获得的结果进行了比较与经典的分光光度法用于运行有色物质的校准曲线所提供的。化学成像技术是相对简单和负担得起的;事实上，它可以用商业上可获得的数码相机和高度用户友好的公共领域软件ImageJ来实现，该软件由美国国立卫生研究院开发并可在互联网上免费下载。所提出的方法在理论上可以用于适应任何经典的比色方法。在一个日益复杂的设备占主导地位的世界中，这种分析方法依赖于简单的osolidaryo分析化学原理;事实上，它可以在无法负担购买和维护温度计的情况下促进定量测定。
An analytical imaging approach to the quantitation of dissolved chemical species absorbed in the visible spectral region is reported. The approach uses digital images of a series of standard solutions of the target substance and their processing with appropriate software to assign a numeric value to each standard in terms of color intensity. Such a value is directly proportional to the analyte concentration and allows the construction of a calibration graph. The results thus obtained are compared with those provided by the classical spectrophotometric method used to run calibration curves for colored substances. The chemical imaging technique is relative simple and affordable; in fact, it can be implemented with a commercially available digital camera and the highly user-friendly public domain software ImageJ, developed by the National Institutes of Health and freely available for download on the Internet. The proposed approach can in theory be used to adapt any classic colorimetric method. In a world dominated by increasingly sophisticated equipment, this analytical procedure relies on straightforward, osolidaryo analytical chemical principles; in fact, it can facilitate quantitative determinations wherever the purchase and maintenance of spectrophotometers is unaffordable.