ISOLATION AND CHARACTERIZATION OF 3 CA-2+-DEPENDENT BETA-GALACTOSIDE-SPECIFIC LECTINS FROM SNAKE-VENOMS
ISOLATION AND CHARACTERIZATION OF 3 CA-2+-DEPENDENT BETA-GALACTOSIDE-SPECIFIC LECTINS FROM SNAKE-VENOMS
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DOI：
10.1042/bj2240301
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发表时间：
1984-01-01
影响因子：
4.1
通讯作者：
OGILVIE, ML
中科院分区：
文献类型：
--
作者：
GARTNER, TK;OGILVIE, ML
Three lactose-inhibited lectins from the venoms of the snakes Agkistrodon contortrix contortrix (southern copperhead), Ancistrodon piscivorus leukostoma (western cottonmouth moccasin) and Crotalus atrox (western diamondback rattlesnake) were isolated and newly characterized. The 3 lectins are similar to thrombolectin, a lectin isolated from the venom of Bothrops atrox (fer-de-lance) with regard to sugar specificity, MW, Ca2+ requirements and sensitivity to reducing agents. Each lectin is a dimer (MW 28,000) consisting of monomers (MW 14,000) indistinguishable on sodium dodecyl sulfate/polyacrylamide gel electrophoresis. Hemagglutination activity is dependent on the presence of Ca2+ and is inhibited by reducing agents. The lectins are not identical and can be distinguished on the basis of relative affinities for inhibiting sugars, isoelectric points and immunoprecipitation assays using anti-(cottonmouth lectin) serum.
