Dual channel optical tomographic imaging of leukocyte recruitment and protease activity in the healing myocardial infarct
Dual channel optical tomographic imaging of leukocyte recruitment and protease activity in the healing myocardial infarct
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DOI：
10.1161/01.res.0000265064.46075.31
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发表时间：
2007-04-27
影响因子：
20.1
通讯作者：
Weissleder, Ralph
中科院分区：
文献类型：
--
作者：
Nahrendorf, Matthias;Sosnovik, David E.;Weissleder, Ralph
心肌梗死后的炎症反应深刻影响组织修复。然而，缺乏有效的工具来连续和无创地评估梗死后炎症的细胞和分子功能。在这里，我们使用多通道荧光分子断层扫描（FMT）对小鼠梗死中巨噬细胞和中性粒细胞介导的吞噬和蛋白水解活动进行时空分辨率分析。我们分别在野生型和FXIII-/-小鼠中进行FMT成像来比较有效愈合和受损愈合的过程。冠状动脉结扎小鼠同时注射Prosense-680和CLIO-VT750，前者是一种报告组织蛋白酶活性的可激活荧光传感器，后者是一种用于成像吞噬细胞募集的磁荧光纳米颗粒。在FMT上，Prosense-680梗死信号比背景高19倍(P
Inflammatory responses after myocardial infarction profoundly impact tissue repair. Yet, efficient tools to serially and noninvasively assess cellular and molecular functions in postinfarct inflammation are lacking. Here we use multichannel fluorescent molecular tomography (FMT) for spatiotemporal resolution of phagocytic and proteolytic activities mediated by macrophages and neutrophils in murine infarcts. We performed FMT imaging to compare the course of efficient and impaired healing in wild-type and FXIII-/- mice, respectively. Mice subjected to coronary ligation received simultaneous injections with Prosense-680, an activatable fluorescence sensor reporting on cathepsin activity, and CLIO-VT750, a magneto-fluorescent nanoparticle for imaging of phagocyte recruitment. On FMT, Prosense-680 infarct signal was 19-fold higher than background (P