Oxidation of NADH by vanadate plus phenylmethanesulfonyl fluoride (PMSF).
Oxidation of NADH by vanadate plus phenylmethanesulfonyl fluoride (PMSF).
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钒酸盐加苯甲磺酰氟 (PMSF) 氧化 NADH。
DOI：
10.7888/juoeh.10.59
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发表时间：
1988
期刊：
影响因子：
--
通讯作者：
M. Kawamura
中科院分区：
文献类型：
--
作者：
K. Takeda;M. Kawamura
苯甲磺酰氟（PMSF）使钒酸盐催化氧化NADH。超氧化物歧化酶和锰抑制氧化，表明超氧阴离子起着至关重要的作用。由于PMSF广泛用于保护酶在纯化时免受蛋白水解裂解，因此在评价钒酸盐对酶的毒性（其活性通过NADH的氧化来监测）或纯化和测定钒酸盐依赖性NADH氧化酶时应予以注意。
Phenylmethanesulfonyl fluoride (PMSF) enabled vanadate to catalyze the oxidation of NADH. Superoxide dismutase and manganese inhibited the oxidation, suggesting that superoxide anion played a crucial role. Since PMSF is widely used to protect enzymes from proteolytic cleavage when they are purified, attention should be paid when the toxicity of vanadate on the enzyme, whose activities are monitored by the oxidation of NADH, is evaluated or when vanadate dependent NADH oxidase is purified and assayed.