Restricted expression of somatostatin receptor 3 to primary cilia in the pancreatic islets and adenohypophysis of mice
Restricted expression of somatostatin receptor 3 to primary cilia in the pancreatic islets and adenohypophysis of mice
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DOI：
10.2220/biomedres.32.73
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发表时间：
2011-02-01
影响因子：
1.2
通讯作者：
Takahashi-Iwanaga, Hiromi
中科院分区：
文献类型：
--
作者：
Iwanaga, Toshihiko;Miki, Takashi;Takahashi-Iwanaga, Hiromi
初级纤毛现在被认为是作为一个基本的，而不是基本的，结构的机械和化学传感的个别细胞。神经元中的初级纤毛表达III型腺苷酸环化酶（ACIII）和生长抑素（生长抑素受体3，SSTR 3）、5-羟色胺和黑色素浓缩激素的GPCR。目前的免疫组织化学和电子显微镜研究显示，在胰岛素和生长激素分泌细胞的小鼠的SSTR 3表达孤纤毛的丰富的发生。SSTR 3的免疫反应性仅限于在这两种细胞类型的纤毛的质膜，不同于以前报道的SSTR的细胞体的免疫组织化学定位。胰岛细胞的初级纤毛比垂体细胞的初级纤毛长，并在细胞间小管内延伸较长距离，细胞间小管内有微绒毛。没有其他内分泌器官提供与SSTR 3表达的初级纤毛，而在这些器官中的初级纤毛经常免疫标记的ACIII抗体。由于生长抑素对胰岛素和GH释放的抑制作用主要受SSTR 1和SSTR 5的调节，因此表达SSTR 3的初级纤毛可能参与了一种不同于通过细胞体中表达的其他SSTR亚型的信号传导。
The primary cilium is now considered to function as a fundamental, not rudimentary, structure for mechanical and chemical sensing by individual cells. Primary cilia in neurons express type III adenylyl cyclase (ACIII) and GPCRs for somatostatin (somatostatin receptor 3, SSTR3), serotonin, and melanin-concentrating hormone. The present immunohistochemical and electron microscopic study revealed an abundant occurrence of SSTR3-expressing solitary cilia in insulin- and growth hormone-secreting cells of the mouse. The SSTR3 immunoreactivity was restricted to the plasma membrane of cilia in both cell types, differing from previously reported immunohistochemical localization of SSTRs to cell bodies. The primary cilia in the islet cells were longer than those in the pituitary cells and extended for a long distance in the intercellular canalicules endowed with microvilli. No other endocrine organs were provided with the SSTR3-expressing primary cilia, while the primary cilia in these organs were frequently immunolabeled with ACIII antibody. Since the somatostatin inhibition of both insulin and GH release is regulated mainly by SSTR1 and SSTR5, the primary cilia expressing SSTR3 may be involved in a signaling which differs from that via other SSTR subtypes expressing in cell bodies.