Bioactive proteins delivery through core-shell nanofibers for meniscal tissue regeneration
Bioactive proteins delivery through core-shell nanofibers for meniscal tissue regeneration
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DOI：
10.1016/j.nano.2019.102090
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发表时间：
2020-01-01
影响因子：
5.4
通讯作者：
D'Lima, Darryl D.
中科院分区：
文献类型：
--
作者：
Baek, Jihye;Lee, Emily;D'Lima, Darryl D.
用纳米纤维支架模拟半月板的超微结构形态，再加上控制生长因子递送到适当的细胞，可以帮助工程组织在体内植入后具有生长、成熟和再生的潜力。我们采用静电纺丝纳米纤维封装血小板衍生生长因子 (PDGF-BB)，这是一种有效的有丝分裂原和化学引诱剂，位于聚乳酸外壳内的血清白蛋白核心中。我们通过在聚乳酸壳中添加水溶性聚乙二醇作为致孔剂来控制局部PDGF-BB的释放。新型核壳纳米纤维生成的 3D 支架具有互连的大孔结构、适当的机械性能和高细胞相容性。掺入 PDGF-BB 增加了细胞活力、增殖和浸润，并上调了人半月板和滑膜细胞中参与半月板细胞外基质合成的关键基因。我们的结果支持了这样的概念：这些核壳纳米纤维可以创造有利于细胞的纳米环境，并可以作为生物活性因子持续释放的系统。 (c) 2019 Elsevier Inc. 保留所有权利。
Mimicking the ultrastructural morphology of the meniscus with nanofiber scaffolds, coupled with controlled growth-factor delivery to the appropriate cells, can help engineer tissue with the potential to grow, mature, and regenerate after in vivo implantation. We electrospun nanofibers encapsulating platelet-derived growth factor (PDGF-BB), which is a potent mitogen and chemoattractant in a core of serum albumin contained within a shell of polylactic acid. We controlled the local PDGF-BB release by adding water-soluble polyethylene glycol to the polylactic acid shell to serve as a porogen. The novel core-shell nanofibers generated 3D scaffolds with an interconnected macroporous structure, with appropriate mechanical properties and with high cell compatibility. Incorporating PDGF-BB increased cell viability, proliferation, and infiltration, and upregulated key genes involved in meniscal extracellular matrix synthesis in human meniscal and synovial cells. Our results support proof of concept that these core-shell nanofibers can create a cell-favorable nanoenvironment and can serve as a system for sustained release of bioactive factors. (c) 2019 Elsevier Inc. All rights reserved.