Upregulation of Aedes aegypti Vago1 by Wolbachia and its effect on dengue virus replication
Upregulation of Aedes aegypti Vago1 by Wolbachia and its effect on dengue virus replication
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DOI：
10.1016/j.ibmb.2017.11.008
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发表时间：
2018-01-01
影响因子：
3.8
通讯作者：
Asgari, Sassan
中科院分区：
文献类型：
--
作者：
Asad, Sultan;Parry, Rhys;Asgari, Sassan
登革热感染及其相关疾病对人类健康构成重大威胁。造成这种感染的病原体是登革病毒(DENV)，它主要通过埃及伊蚊的叮咬传播给人类。由于无法获得有效的疫苗，最近引发了旨在控制病媒的新一轮研究努力。到目前为止，沃尔巴克氏杆菌作为一种内共生细菌，已经显示出作为一种新的生防剂的希望，以限制DENV在载体中的复制，尽管潜在的抗病毒机制仍然不清楚。最近的研究表明，Vago作为一种新的分泌蛋白，参与致倦库蚊天然免疫途径之间的串扰，从而限制西尼罗河病毒的复制，具有潜在的作用。在本研究中，我们鉴定了Ae中Vago蛋白的两个同源物。埃及伊蚊，并研究了它们在沃尔巴克氏菌wMelPop菌株感染的情况下的调节。此外，我们还研究了沃尔巴克氏菌高度诱导的AeVagol在沃尔巴克氏菌-蚊子-DENV相互作用中的作用。对沃尔巴克氏菌感染细胞中AeVagol基因的敲除研究导致DENV复制显著增加，而对沃尔巴克氏菌密度没有影响。我们的结果表明，沃尔巴克氏菌在Ae.埃及伊蚊可能作为宿主因子抑制蚊子体内的DENV复制。(C)2017爱思唯尔有限公司。保留所有权利。
Dengue infection along with its related disease conditions poses a significant threat to human health. The pathogen responsible for this infection is dengue virus (DENV) which is primarily transmitted to humans through the bites of Aedes aegypti mosquitoes. Unavailability of a potent vaccine has recently sparked renewed research endeavours aimed at vector control. To date, Wolbachia as an endosymbiotic bacterium has shown promise as a novel biocontrol agent to restrict DENV replication in the vector, although the underlying antiviral mechanism remains elusive. Recent studies have demonstrated the potential role of Vago as a novel secretory protein involved in cross-talk between the innate immune pathways in Culex quinquefasciatus mosquitoes to restrict West Nile virus replication. In this study, we have identified two homologs of the Vago protein in Ae. aegypti and looked into their modulation in the case of Wolbachia wMelPop strain infection. Furthermore, we have investigated the role of AeVagol, that is highly induced by Wolbachia, in the context of Wolbachia-mosquito-DENV interactions. Knockdown studies of the AeVagol gene in Wolbachia-infected cells led to significant increases in DENV replication, with no effect on Wolbachia density. Our results suggest that the Wolbachia-induced AeVagol in Ae. aegypti may function as a host factor to suppress DENV replication in the mosquito. (C) 2017 Elsevier Ltd. All rights reserved.