Expression of differentiation and murine leukemia virus antigens on cells of primary tumors and cell lines derived from chemically induced lymphomas of RF/J mice.
Expression of differentiation and murine leukemia virus antigens on cells of primary tumors and cell lines derived from chemically induced lymphomas of RF/J mice.
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RF/J 小鼠原发性肿瘤细胞和化学诱导淋巴瘤细胞系中分化抗原和鼠白血病病毒抗原的表达。
DOI：
10.1073/pnas.81.23.7612
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发表时间：
1984
影响因子：
11.1
通讯作者：
Lilly,F
中科院分区：
文献类型：
--
作者：
Goodenow,MM;Lilly,F
3-甲基胆蒽（MCA）皮肤涂抹快速诱导RF/J小鼠胸腺淋巴瘤的发病率为100%。肿瘤仅在T淋巴细胞谱系的细胞中发展，并显示表面表型：Thy-1+、Lyt-1+、Lyt-2+、Qa-1+、H-2K+、H-2D+、TL-、IG-、Ia-。这种表型类似于可的松耐药的髓质胸腺细胞，而自发性AKR胸腺瘤的表型类似于可的松敏感的皮质胸腺细胞。表型差异很小，从一个肿瘤到另一个，并保持在体内同系传代和适应培养生长。感染性嗜亲性小鼠白血病病毒（MuLV）在少数原发性肿瘤和细胞系中产生，但没有可靠地检测到异嗜性或貂细胞病灶诱导病毒（MCF）MuLV的产生。正常胸腺细胞上MuLV相关抗原表达以年龄依赖性方式增加，胸腺瘤细胞上的水平与年龄匹配的正常对照胸腺细胞上的水平相似。MuLV非生产性肿瘤细胞表达很少或没有AKR亲嗜性gp 70的表位，但当用针对MCFenv糖蛋白制备的抗血清进行测试时，它们呈阳性。
3-Methylcholanthrene (MCA) skin painting rapidly induced a 100% incidence of thymic lymphomas in RF/J mice. Tumors developed exclusively in cells of the T-lymphocyte lineage and displayed the surface phenotype: Thy-1+, Lyt-1+, Lyt-2+, Qa-1+, H-2K+, H-2D+, TL-, Ig-, Ia-. This phenotype resembled that of cortisone-resistant, medullary thymocytes, whereas the phenotype of spontaneous AKR thymomas resembles that of cortisone-sensitive, cortical thymocytes. The phenotype differed very little from one tumor to another and was maintained during syngeneic passage in vivo and adaptation to growth in culture. Infectious ecotropic murine leukemia virus (MuLV) was produced in a minority of primary tumors and cell lines, but no xenotropic or mink cell focus-inducing virus (MCF) MuLV production was reliably detected. MuLV-related antigen expression on normal thymocytes increased in an age-dependent manner, and levels on thymoma cells were similar to those on thymocytes from age-matched normal controls. MuLV nonproducer tumor cells expressed few or no epitopes of AKR ecotropic gp70, but they were positive when tested with an antiserum prepared against MCFenv glycoprotein.