Spatially regulated translation in embryos:: Asymmetric expression of maternal Wnt-11 along the dorsal-ventral axis in Xenopus
Spatially regulated translation in embryos:: Asymmetric expression of maternal Wnt-11 along the dorsal-ventral axis in Xenopus
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DOI：
10.1006/dbio.1999.9426
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发表时间：
1999-10-15
影响因子：
2.7
通讯作者：
Yost, HJ
中科院分区：
文献类型：
--
作者：
Schroeder, KE;Condic, NL;Yost, HJ
从对称到不对称的转变是细胞和发育生物学的中心主题。在非洲爪蟾胚胎中，背腹不对称是由受精后第一个细胞周期期间微管依赖性细胞质旋转引发的。在这里，我们表明，细胞质旋转启动母体 Xwnt-11 RNA 的差异细胞质多腺苷酸化，编码细胞间信号因子 Wnt 家族的成员。 Xwnt-11 mRNA 沿背腹轴的翻译调节导致 Xwnt-11 蛋白的不对称积累。这些结果证明了母体细胞信号因子沿脊椎动物背腹轴的空间调节翻译，并代表了 Wnt 基因调节的新机制。母体 RNA 翻译的空间调节已在无脊椎动物中建立，似乎是细胞内不对称性的产生和随后的多细胞体模式形成的进化上保守的机制。 (C) 1999 年学术出版社。
Transition from symmetry to asymmetry is a central theme in cell and developmental biology. In Xenopus embryos, dorsal-ventral asymmetry is initiated by a microtubule-dependent cytoplasmic rotation during the first cell cycle after fertilization. Here we show that the cytoplasmic rotation initiates differential cytoplasmic polyadenylation of maternal Xwnt-11 RNA, encoding a member of the Wnt family of cell-cell signaling factors. Translational regulation of Xwnt-11 mRNA along the dorsal-ventral axis results in asymmetric accumulation of Xwnt-11 protein. These results demonstrate spatially regulated translation of a maternal cell-signaling factor along the vertebrate dorsal-ventral axis and represent a novel mechanism for Wnt gene regulation. Spatial regulation of maternal RNA translation, which has been established in invertebrates, appears to be an evolutionarily conserved mechanism in the generation of intracellular asymmetry and the consequential formation of the multicellular body pattern. (C) 1999 Academic Press.