Engineering of SH2 Domains for the Recognition of Protein Tyrosine O-Sulfation Sites.
Engineering of SH2 Domains for the Recognition of Protein Tyrosine O-Sulfation Sites.
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用于识别蛋白质酪氨酸 O-硫酸化位点的 SH2 结构域工程。
DOI：
10.1007/978-1-0716-3393-9_16
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发表时间：
2023
期刊：
影响因子：
--
通讯作者：
Guo,Jiantao
中科院分区：
文献类型：
--
作者：
Waldrop,SeanPaul;Niu,Wei;Guo,Jiantao
蛋白质工程给工业过程、生物材料、纳米技术、生物传感器和生物医学应用带来了进步。本章将侧重于设计Src同源2结构域(SH2)，作为识别含有硫代酪氨酸的多肽或蛋白质的抗体模拟物。与抗硫酪氨酸抗体相比，SH2突变体体积小得多，可以低成本大量异源表达和纯化。本章将描述如何使用噬菌体展示技术来鉴定硫代酪氨酸结合SH2突变体，以及随后在复杂生物样品中富含硫代酪氨酸的多肽。
Protein engineering has brought advances to industrial processes, biomaterials, nanotechnology, biosensors, and biomedical applications. This chapter will focus on the engineering of Src Homology 2 domains (SH2) to act as an antibody mimetic for the recognition of sulfotyrosine-containing peptides or proteins. In comparison to anti-sulfotyrosine antibodies, SH2 mutants have much smaller size and can be heterologously expressed and purified in large quantity at low cost. This chapter will describe the use of phage display to identify a sulfotyrosine-binding SH2 mutant and the subsequent enrichment of sulfotyrosine-containing peptides in complex biological samples.