Miniscope GRIN Lens System for Calcium Imaging of Neuronal Activity from Deep Brain Structures in Behaving Animals.
Miniscope GRIN Lens System for Calcium Imaging of Neuronal Activity from Deep Brain Structures in Behaving Animals.
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DOI：
10.1002/cpns.56
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发表时间：
2019-01
影响因子：
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通讯作者：
Lin DT
中科院分区：
文献类型：
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作者：
Zhang L;Liang B;Barbera G;Hawes S;Zhang Y;Stump K;Baum I;Yang Y;Li Y;Lin DT
通过微型荧光显微镜(Miniscope)系统显示自由行为动物大脑深层的神经活动，对于理解认知功能背后的神经编码机制变得越来越重要。在这里，我们介绍了我们的定制设计的迷你望远镜梯度折射率(GRIN)透镜系统，可以同时记录数百个神经元数月。该方案包括微型望远镜的设计，GRIN晶状体植入的手术程序，安装在小鼠头部的微型望远镜，以及数据采集和分析。首先，用表达GCaMP6的病毒标记靶脑区；其次，在靶脑区上方植入GRIN晶状体；第三，在小鼠手术康复后，将迷你望远镜安装在小鼠头部GRIN晶状体上方；最后，记录行为自由的小鼠的神经活动。该系统可用于纵向记录相同数量的神经元，从而能够阐明行为控制的神经机制。
Visualizing neural activity from deep brain regions in freely behaving animals through miniature fluorescent microscope (miniscope) systems is becoming more important for understanding neural encoding mechanisms underlying cognitive functions. Here we present our custom-designed miniscope GRadient INdex (GRIN) lens system that enables simultaneously recording from hundreds of neurons for months. This protocol includes miniscope design, the surgical procedure for GRIN lens implantation, miniscope mounting on the head of a mouse, and data acquisition and analysis. First, a target brain region is labeled with virus expressing GCaMP6; Second, a GRIN lens is implanted above the target brain region; Third, following mouse surgical recovery, a miniscope is mounted on the head of the mouse above the GRIN lens; Finally, neural activity is recorded from the freely behaving mouse. This system can be applied to recording the same population of neurons longitudinally, enabling the elucidation of neural mechanisms underlying behavioral control.