Asymmetric requirement for cholesterol in receptor-bearing but not envelope-bearing membranes for fusion mediated by ecotropic murine leukemia virus
Asymmetric requirement for cholesterol in receptor-bearing but not envelope-bearing membranes for fusion mediated by ecotropic murine leukemia virus
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DOI：
10.1128/jvi.76.13.6701-6709.2002
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发表时间：
2002-07-01
影响因子：
5.4
通讯作者：
Silver, J
中科院分区：
文献类型：
--
作者：
Lu, XB;Xiong, Y;Silver, J
我们发现亲嗜性小鼠白血病病毒包膜介导的融合依赖于受体膜中的胆固醇。昆虫细胞中的效果比哺乳动物细胞大10倍以上，可能是因为前者可以更广泛地消耗胆固醇。胆固醇显然是不需要的脂肪细胞轴承膜的事实表明，它在膜融合的不对称步骤中发挥作用，并反对一类模型，其中胆固醇是重要的对称融合中间体。昆虫细胞系统有望阐明膜筏在细胞生理学其他方面的作用。
We show that fusion mediated by ecotropic murine leukemia virus envelope is dependent on cholesterol in receptor-bearing membranes. The effect is >10 times larger in insect cells than mammalian cells, probably because the former can be more extensively depleted of cholesterol. The fact that cholesterol is apparently not needed in envelope-bearing membranes suggests that it plays a role in an asymmetric step in membrane fusion and argues against a class of models in which cholesterol is important in symmetric fusion intermediates. The insect cell system has promise for clarifying the role of membrane rafts in other aspects of cell physiology.