A novel chloroplast-localized protein EMB1303 is required for chloroplast development in Arabidopsis
A novel chloroplast-localized protein EMB1303 is required for chloroplast development in Arabidopsis
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拟南芥叶绿体发育需要一种新型叶绿体定位蛋白 EMB1303
DOI：
10.1038/cr.2009.84
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发表时间：
2009-10-01
期刊：
影响因子：
44.1
通讯作者：
Yang, Shuhua
中科院分区：
文献类型：
--
作者：
Huang, Xiaozhen;Zhang, Xiaoyan;Yang, Shuhua
为了了解叶绿体发育的分子机制，我们分离并鉴定了拟南芥白化突变体emb1303-1。该突变体在含蔗糖的合成培养基上表现出严重的矮化表型，白化莲座叶小，根短。它缺乏色素，在土壤中生长时幼苗致命。突变体的胚胎发育延迟，但种子萌发没有明显受损。emb1303-1的质体在早期发育阶段被阻滞，没有典型的类囊体膜堆叠。遗传和分子分析发现，EMB1303基因编码一种新的叶绿体定位蛋白。微阵列和RT-PCR分析显示，在突变体中，一些参与光合作用和叶绿体生物发生的核和质体编码基因大幅下调。此外，几种主要叶绿体蛋白的积累在emb1303-1中受到严重损害。这些结果表明，EMB1303对叶绿体发育至关重要。
To understand the molecular mechanisms underlying chloroplast development, we isolated and characterized the albino mutant emb1303-1 in Arabidopsis. The mutant displayed a severe dwarf phenotype with small albino rosette leaves and short roots on a synthetic medium containing sucrose. It is pigment-deficient and seedling lethal when grown in soil. Embryo development was delayed in the mutant, although seed germination was not significantly impaired. The plastids of emb1303-1 were arrested in early developmental stages without the classical stack of thylakoid membrane. Genetic and molecular analyses uncovered that the EMB1303 gene encodes a novel chloroplast-localized protein. Microarray and RT-PCR analyses revealed that a number of nuclear-and plastid-encoded genes involved in photosynthesis and chloroplast biogenesis were substantially downregulated in the mutant. Moreover, the accumulation of several major chloroplast proteins was severely compromised in emb1303-1. These results suggest that EMB1303 is essential for chloroplast development.