A comparison of marker-assisted and phenotypic selection for high grain protein content in spring wheat
A comparison of marker-assisted and phenotypic selection for high grain protein content in spring wheat
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DOI：
10.1007/s10681-006-9185-5
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发表时间：
2006-11-01
期刊：
影响因子：
1.9
通讯作者：
Leach, Gene D.
中科院分区：
文献类型：
--
作者：
Davies, John;Berzonsky, William A.;Leach, Gene D.
小麦籽粒蛋白质含量（GPC）是决定硬春小麦（Triticum aestivum L.）最终品质的主要因素，分子标记辅助选择（MAS）可以帮助育种者培育高GPC的品种。使用通过将低GPC品种（Ember）和（McVey）与（Glupro）杂交而开发的两个群体进行两个实验，其中（Glupro）含有来自二粒小麦（DIC）的高GPC QTL。在一个实验中，采用MAS和表型选择（PS）来选择高GPC基因型，并且使所选择的基因型在六个北达科他州（ND）、美国环境中生长。在第二个实验中，使用分子标记从每个群体的DIC等位基因的每个标记类别中选择BC2F2植物。将这些植物自花授粉两次以产生BC2F4植物，其在单一ND和Minnesota（MN）环境中生长。在两种环境下，PS处理的株系的平均GPC最高，而在其他四种环境下，MAS和PS处理的株系之间的平均GPC差异不显著。推测DIC等位基因纯合的品系的GPC显著高于其各自的低GPC亲本。由标记（r（2））解释的表型GPC变异在ND为30%，在MN环境为15%。在选择高GPC基因型方面，PS的使用与MAS一样有效，并且在某些环境中更有效。这可能归因于PS使得能够选择主要QTL和有助于GPC的其他基因。利用分子标记可能更有利于在亲本开发过程中的回交程序中转移高GPC DIC QTL。
Wheat grain protein content (GPC) is a primary end-use quality determinant for hard spring wheat (Triticum aestivum L.), and marker-assisted selection (MAS) could help plant breeders to develop high GPC cultivars. Two experiments were conducted using two populations developed by crossing low GPC cultivars (Ember) and (McVey) with (Glupro), which contains a high GPC QTL from Triticum dicoccoides (DIC). In one experiment, MAS and phenotypic selection (PS) were employed to select high GPC genotypes, and the selected genotypes were grown in six North Dakota (ND), USA environments. In a second experiment, molecular markers were used to select BC2F2 plants from each marker class for the DIC allele from each population. These plants were twice self-pollinated to produce BC2F4 plants, which were grown in single ND and Minnesota (MN) environments. Mean GPC was highest among lines using PS at two environments and not significantly different between MAS and PS in the other four environments. Lines presumably homozygous for DIC alleles had significantly higher GPC than their respective low GPC parents. The phenotypic GPC variation explained by the markers (r(2)) was 30% at the ND and 15% at the MN environment. The use of PS was as effective as MAS in selecting for high GPC genotypes and more effective in some environments. This likely can be attributed to PS enabling selection for both the major QTL and other genes contributing to GPC. The use of molecular markers might be more advantageous for transferring the high GPC DIC QTL in a backcrossing program during parent development.