DETERMINATION OF THE ORIENTATION DISTRIBUTION OF ADSORBED FLUOROPHORES USING TIRF .1. THEORY
DETERMINATION OF THE ORIENTATION DISTRIBUTION OF ADSORBED FLUOROPHORES USING TIRF .1. THEORY
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DOI：
10.1016/s0006-3495(95)80439-x
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发表时间：
1995-06-01
影响因子：
3.4
通讯作者：
KLEIJN, JM
中科院分区：
文献类型：
--
作者：
BOS, MA;KLEIJN, JM
全内反射荧光光谱技术可用于测定被吸附的荧光分子的取向。对于荧光基团是卟啉环的情况，基本的理论被概括和详细地阐述。结果表明，如果同时测量荧光强度及其偏振作为入射激光偏振的函数，就可以得到取向分布的有序参数。由这些序参数可以用最大熵方法得到取向分布的近似值。
The spectroscopic technique total internal reflection fluorescence can be used for determination of the orientation of adsorbed fluorescent molecules. The underlying theory is presented in general terms and elaborated in detail for the case that the fluorescent group is a porphyrin ring. It is shown that order parameters of the orientation distribution can be obtained if both the fluorescence intensity and its polarization are measured as functions of the polarization of the incident laser beam. From these order parameters an approximation of the orientation distribution can be derived by the maximum-entropy method.