Regulation of Na,K-adenosine triphosphatase gene expression by sodium ions in cultured neonatal rat cardiocytes.
Regulation of Na,K-adenosine triphosphatase gene expression by sodium ions in cultured neonatal rat cardiocytes.
复制标题
培养的新生大鼠心肌细胞中钠离子对Na,K-腺苷三磷酸酶基因表达的调节。
DOI：
10.1172/jci116781
复制
发表时间：
1993
期刊：
影响因子：
--
通讯作者：
Y. Hara
中科院分区：
文献类型：
--
作者：
Keiji Yamamoto;Uichi Ikeda;Y. Seino;Y. Tsuruya;A. Oguchi;K. Okada;S. Ishikawa;T. Saito;Kiyoshi Kawakami;Y. Hara
Na,K-ATPase (Na,K-pump) plays an important role in the regulation of intracellular ion composition. The purpose of this study is to determine whether Na+ regulates the levels of mRNA coding for Na,K-ATPase alpha and beta subunits in cultured neonatal rat cardiocytes. We measured intracellular Na+ levels ([Na+]i) in cardiocytes using a Na(+)-sensitive fluorescence dye (SBFI). 1 mM ouabain caused a significant increase in [Na+]i in cardiocytes; from 12.8 +/- 0.3 to 28.8 +/- 1.8 mM. Exposure of cardiocytes to 1 mM ouabain resulted in a three- to fourfold increase in alpha 1, alpha 2, and alpha 3 mRNA accumulation, and an approximate two-fold increase in beta 1 mRNA accumulation. A maximum elevation was reached at 60 min in both cases. The ouabain-induced alpha 1 mRNA accumulation was still observed in the Ca(2+)-free culture medium. Exposure of cardiocytes to 10 microM monensin in the absence of extracellular Ca2+ also resulted in a threefold increase in alpha 1 mRNA accumulation. The increased alpha 1 mRNA expression by 1 mM ouabain was associated with a fourfold increase in alpha 1 subunit protein accumulation. Transfection experiments with chimeric plasmids containing 5'-flanking sequences of alpha 1, alpha 2, and alpha 3 isoform genes and a luciferase reporter gene revealed that 1 mM ouabain caused a twofold increase in luciferase activity in each alpha system. These results suggest that Na+ directly regulates Na,K-ATPase gene expression in cardiocytes. The transfection study further supports the premise that Na(+)-responsive elements are located within the 5'-flanking sequences of each alpha isoform gene.
登录
查看更多内容
DOI：
10.1016/s0021-9258(17)42732-3
发表时间：
1984-08
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
R. Farley;C. M. Tran;C. Carilli;D. Hawke;J. Shively
通讯作者：
R. Farley;C. M. Tran;C. Carilli;D. Hawke;J. Shively
醛固酮介导的成年和新生大鼠心肌细胞中 Na , K( )-ATP 酶基因表达的调节。
DOI：
--
发表时间：
1991
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
Ikeda,U;Hyman,R;Smith,TW;Medford,RM
通讯作者：
Medford,RM
鉴定 Na,K-ATP 酶 β 亚基的推定亚型。
DOI：
--
发表时间：
1989
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
Martin-Vasallo,P;Dackowski,W;Emanuel,JR;Levenson,R
通讯作者：
Levenson,R
DOI：
10.1016/s0021-9258(19)47321-3
发表时间：
1989-11
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
Akwasi MintaS;Roger Y. TsienO
通讯作者：
Akwasi MintaS;Roger Y. TsienO
[Ca2]i 调节 Na /K( )-ATPase α 1 亚基的转录速率。
DOI：
--
发表时间：
1991
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
Rayson,BM
通讯作者：
Rayson,BM