Degradation of terbuthylazine, difenoconazole and pendimethalin pesticides by selected fungi cultures
Degradation of terbuthylazine, difenoconazole and pendimethalin pesticides by selected fungi cultures
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DOI：
10.1016/j.scitotenv.2012.07.027
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发表时间：
2012-10-01
影响因子：
9.8
通讯作者：
Caldeira, A. T.
中科院分区：
文献类型：
--
作者：
Pinto, A. P.;Serrano, C.;Caldeira, A. T.
水体被杀虫剂等外来化合物污染是一个严重的环境问题，目前大量的杀虫剂正在污染欧洲的水资源。研究了尖孢镰刀菌、米曲霉、香菇、短密青霉和萨克森链霉菌在间歇液体培养条件下对农药特丁津、苯醚甲环唑和二甲双灵的降解能力。这些农药是常见的土壤和水污染物，特丁津被认为是地表环境中最持久的三嗪除草剂。通过对能够代谢所研究农药的真菌的浓缩、分离和筛选，获得了短密拟青霉和腐生拟青霉。这些菌株是从两种农药引发的材料(土壤和生物混合物)中获得的。尽管特丁津的持久性相对较高，但本工作的结果表明，与其他类似研究的结果相比，所研究的真菌物种具有较高的生物转化这种外来物质的能力。米曲霉对特丁肼的降解率最高(接近80%)，但对特丁肼的主要生物降解率仍以短拟青霉为主。短毛拟青霉代谢特丁津的较高能力可能是通过长期暴露在除草剂污染中而获得的。腐乳杆菌在间歇液体培养中可以去除99.5%的二甲戊灵。臭菇是一种对农药，特别是苯醚甲环唑和二甲戊灵具有很高生物降解力的真菌。此外，在臭菇液体培养基中检测到代谢物去乙基特丁肼，表明该真菌可以降解特丁肼。所研究的真菌可以被证明是有效的农药降解菌，提高了含有所研究的外源化合物或具有相似固有特性的化合物污染的废水的生物净化系统的效率。(C)2012爱思唯尔B.V.保留所有权利。
Contamination of waters by xenobiotic compounds such as pesticides presents a serious environmental problem with substantial levels of pesticides now contaminating European water resources. The aim of this work was to evaluate the ability of the fungi Fusarium oxysporum, Aspergillus oryzae, Lentinula edodes, Penicillium brevicompactum and Lecanicillium saksenae, for the biodegradation of the pesticides terbuthylazine, difenoconazole and pendimethalin in batch liquid cultures. These pesticides are common soil and water contaminants and terbuthylazine is considered the most persistent triazine herbicide in surface environments. P. brevicompactum and L. saksenae were achieved by enrichment, isolation and screening of fungi capable to metabolize the pesticides studied. The isolates were obtained from two pesticide-primed materials (soil and biomixture).Despite the relatively high persistence of terbuthylazine, the results obtained in this work showed that the fungi species studied have a high capability of biotransformation of this xenobiotic, comparatively the results obtained in other similar studies. The highest removal percentage of terbuthylazine from liquid medium was achieved with A. oryzae (similar to 80%), although the major biodegradation has been reached with P. brevicompactum. The higher ability of P. brevicompactum to metabolize terbuthylazine was presumably acquired through chronic exposure to contamination with the herbicide. L. saksenae could remove 99.5% of the available pendimethalin in batch liquid cultures.L. edodes proved to be a fungus with a high potential for biodegradation of pesticides, especially difenoconazole and pendimethalin.Furthermore, the metabolite desethyl-terbuthylazine was detected in L. edodes liquid culture medium, indicating terbuthylazine biodegradation by this fungus.The fungi strains investigated could prove to be valuable as active pesticide-degrading microorganisms, increasing the efficiency of biopurification systems containing wastewaters contaminated with the xenobiotics studied or compounds with similar intrinsic characteristics. (C) 2012 Elsevier B.V. All rights reserved.