Natural killer cells in Behçet's disease.
Natural killer cells in Behçet's disease.
复制标题
白塞氏病中的自然杀伤细胞。
DOI：
10.1111/j.1365-2249.1994.tb06072.x
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发表时间：
1988
影响因子：
4.6
通讯作者：
J. Touraine
中科院分区：
文献类型：
--
作者：
K. Hamzaoui;K. Ayed;M. Hamza;J. Touraine
我们研究了白塞病（BD）患者在非活动期和恢复期外周血中自然杀伤（NK）细胞的活性和数量，以及健康对照。BD患者活动期辅助/抑制细胞比值（OKT_4/OKT_8）<1.0，恢复期正常。在BD活动期和恢复期，OKT 8+细胞和Leu 7+细胞相对增加。双标记分析显示，活动期患者同时表达T8+和Leu 7+抗原的细胞亚群（T8+/Leu 7+）增加，恢复期正常。在BD患者中评价与Leu 11单克隆抗体反应的细胞（活性NK细胞）的频率。活动期患者外周血T8+/Leu 7+双标记细胞亚群增加，Leu 11+细胞亚群减少，而恢复期患者大多数Leu 7+细胞缺乏OKT 8抗原。Leu 11+细胞在恢复期呈平行增加。该表型分析与来自外周血的细胞群的NK体外功能评价一起进行。BD患者临床活动期NK细胞活性明显低于健康对照组和恢复期患者。活动性BD患者外周血NK功能的降低可能与NK细胞的未成熟形式和/或T8+/Leu 7+细胞百分比的增加有关。
We studied natural killer (NK) cell activity and numbers in the peripheral blood obtained from patients with Behçet's disease (BD) in inactive and convalescent stage, and from healthy controls. Ratios of helper/suppressor cells (OKT4/OKT8) were below 1.0 in patients with active stage and were normal in the convalescent stage of BD. A relative increase of OKT8+ cells and at the same time of Leu 7+ cells was obtained in the active and convalescent BD stages. Double marker analysis revealed that the sub-population of cells expressing both the T8+ and the Leu 7+ antigen (T8+/Leu 7+) was increased in patients with active stage, and normal in the convalescent stage. The frequency of cells reactive with Leu 11 monoclonal antibody (active NK cells) was evaluated in patients with BD. Data from peripheral blood showed an increased sub-population of T8+/Leu 7+ double marker cells, and a decreased Leu 11+ cell sub-population in patients with active BD, but the majority of Leu 7+ cells in patients with convalescent stage lacked OKT8 antigen when investigated in a double marker system. A parallel increase of Leu 11+ cells was observed in the convalescent stage. This phenotypic analysis was carried out with the NK in vitro functional evaluation of cell populations from peripheral blood. NK cell activity in the clinically active stage of BD was significantly lower than that of healthy controls and patients in the convalescent stage. The decrease of peripheral blood NK function in patients with active BD may be related to the presence of immature forms of NK cells and/or to the increased percentage of T8+/Leu 7+ cells.