ION SPRAY INTERFACE FOR COMBINED LIQUID CHROMATOGRAPHY/ATMOSPHERIC PRESSURE IONIZATION MASS-SPECTROMETRY
ION SPRAY INTERFACE FOR COMBINED LIQUID CHROMATOGRAPHY/ATMOSPHERIC PRESSURE IONIZATION MASS-SPECTROMETRY
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DOI：
10.1021/ac00149a003
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发表时间：
1987-11-15
影响因子：
7.4
通讯作者：
HENION, JD
中科院分区：
文献类型：
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作者：
BRUINS, AP;COVEY, TR;HENION, JD
结合离子蒸发和电喷雾的原理，构建了液相色谱/质谱（LC/MS）接口。该装置被称为"离子喷雾" LC/MS接口。来自微孔LC的总流出物通过浮动在±. 3kV，并在大气压下在干燥氮气中分散成带电液滴。由带电液滴发射的离子由能够在大气压力下从区域采样离子的三重四极质谱仪进行质量分析。观察到指示样品分子量的离子，几乎没有或没有碎片。溶液中的多电荷离子在相应的质谱中显示多电荷离子。碰撞诱导解离反应产生对应于结构单元的碎片离子。全扫描检测限似乎是约10纳克的离子或选定的中性化合物和选定的离子监测条件下约10皮克。
An interface for liquid chromatography/mass spectrometry (LC/MS) was constructed that combines the principles of ion evaporation and electrospray. This device is called an "ion spray" LC/MS interface. The total effluent from a microbore LC is fed through a pneumatic nebulizer floating at .+-. 3 kV and dispersed into charged droplets in dry nitrogen at atmospheric pressure. Ions emitted by the charged droplets are mass analyzed by a triple quadrupole mass spectrometer capable of sampling ions from a region at atmospheric pressure. Ions indicating the molecular weight of a sample are observed with little or no fragmentation. Multiply charged ions in solution show multiply charged ions in the corresponding mass spectra. Collision-induced dissociation reactions generate fragment ions corresponding to structural units. The full-scan detection limit appears to be about 10 ng for ionic or selected neutral compounds and about 10 pg under selected ion monitoring conditions.