The Kinesin-1 Chemomechanical Cycle: Stepping Toward a Consensus
The Kinesin-1 Chemomechanical Cycle: Stepping Toward a Consensus
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DOI：
10.1016/j.bpj.2016.02.025
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发表时间：
2016-03-29
影响因子：
3.4
通讯作者：
Hancock, William O.
中科院分区：
文献类型：
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作者：
Hancock, William O.
肌动蛋白-1是理解运动蛋白机械化学基础和解释肌动蛋白超家族功能多样性的模型。尽管在过去三十年中进行了持续的工作，但关于触发步进循环中两个关键转变的事件仍然存在分歧：从微管中脱离尾头和将系住的头结合到下一个微管结合位点。这篇综述描述了这些事件的相互矛盾的观点，并强调了最近的工作，阐明了这些长期存在的争议。最后，它提出了一个共识的动力蛋白-1化学力学，结合了最近的工作，解决了差异，并强调了未来实验工作的关键问题。希望这个模型能提供一个框架，来理解不同的运动蛋白是如何为它们特定的细胞角色而调整的。
Kinesin-1 serves as a model for understanding fundamentals of motor protein mechanochemistry and for interpreting functional diversity across the kinesin superfamily. Despite sustained work over the last three decades, disagreements remain regarding the events that trigger the two key transitions in the stepping cycle: detachment of the trailing head from the microtubule and binding of the tethered head to the next tubulin binding site. This review describes the conflicting views of these events and highlights recent work that sheds light on these long-standing controversies. It concludes by presenting a consensus kinesin-1 chemomechanical that incorporates recent work, resolves discrepancies, and highlights key questions for future experimental work. It is hoped that this model provides a framework for understanding how diverse kinesins are tuned for their specific cellular roles.