Identification of microRNA signatures in umbilical cord blood associated with maternal characteristics
Identification of microRNA signatures in umbilical cord blood associated with maternal characteristics
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DOI：
10.7717/peerj.6981
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发表时间：
2019-05-30
期刊：
影响因子：
2.7
通讯作者：
Slaby, Ondrej
中科院分区：
文献类型：
--
作者：
Juracek, Jaroslav;Piler, Pavel;Slaby, Ondrej
背景。脐带血可以作为有用的血液标记物来源，使产前和新生儿诊断更加有效和可靠。MicroRNAs （miRNAs）在体液中无处不在，它们被用来检测和监测各种生理和病理状况。在这项描述性研究中，我们旨在确定与母体基本体细胞和流行病学特征相关的miRNA表达谱的变化。这项研究基于CELSPAC: TNG（中欧父母和孩子的纵向研究：下一代）研究试点阶段的24位母亲。在分娩时收集脐带血，并使用microRNA Ready-to-use PCR Human Panel I+II TaqMan微阵列进行全球miRNA分析。对其表达谱与母亲年龄、体重指数、孕期体重增加、血型、Rh因子状态、孕期过敏、成瘾物质滥用和吸烟状况的关系进行统计分析。我们分析了24份脐带血样本中752个人类成熟mirna的表达。对于所有测试的母体特征，我们描述了显著解除调控的mirna的特定特征（P < 0.05）。分析显示，有7种miRNA与母亲年龄相关（35岁以下女性中3种miRNA增加，4种miRNA减少），14种miRNA与BMI状态相关（BMI低于25的女性中5种miRNA增加，9种miRNA减少），9种miRNA与怀孕期间母亲体重增加相关（体重增加< 12 kg的女性中8种miRNA增加，1种miRNA减少）。此外，17个mirna与血型相关（2个mirna在A型血中减少，11个mirna在B型血中增加，2个mirna在AB型血中增加，2个mirna在0型血中增加），17个mirna与母亲Rh状态相关。我们还在过敏女性的脐带血中检测到7个mirna失调（过敏女性中4个升高，3个降低），4个mirna与成瘾物质滥用状态相关（成瘾物质滥用女性中2个升高，2个降低），8个mirna与怀孕期间母亲吸烟相关。我们成功地描述了脐带血中与母亲相关的基本特征相关的miRNA谱的差异。我们的数据表明，脐带血中的mirna是可检测的，并且与广泛的母体特征相关。这些结果表明，mirna有可能并且应该作为筛查和诊断妊娠相关并发症和病理的生物标志物进行研究。
Background. Umbilical cord blood could serve as useful source of blood markers enabling more efficient and reliable prenatal and neonatal diagnostics. MicroRNAs (miRNAs) are ubiquitous in body fluids where they were used for detecting and monitoring various physiological and pathological conditions. In this descriptive study, we aimed to identify changes in miRNA expression profiles associated with basic maternal somatic and epidemiological characteristics.Methods. Study is based on 24 mothers from the Pilot phase of CELSPAC: TNG (Central European Longitudinal Studies of Parents and Children: The Next Generation) study. Cord blood was collected at time of delivery and global miRNA profiling was performed using microRNA Ready-to-use PCR Human Panel I+II TaqMan microarrays. Expression profiles were statistically evaluated in relation to maternal age, BMI, pregnancy weight gain, blood type, Rh factor status, allergies during pregnancy, addictive substance abuse and smoking status.Results. We analyzed expression of 752 human mature miRNAs in 24 samples of umbilical cord blood. For all maternal characteristics tested we described a specific signature of significantly deregulated miRNAs (P < 0.05). Analysis revealed seven miRNA associated with maternal age (three increased and four decreased in women younger than 35 years), 14 miRNAs associated with BMI status (five miRNAs increased and nine miRNAs decreased in women with BMI > 25) and nine miRNAs associated with maternal weight gain during pregnancy (eight miRNAs increased, and one miRNA decreased in women with weight gain < 12 kg). Additionally, 17 miRNAs correlated to blood type (two miRNAs decreased in blood type A, 11 increased in blood type B, two miRNAs increased in blood type AB and two miRNAs increased in blood type 0) and 17 miRNAs to Rh status of mother. We also detected seven miRNAs deregulated in umbilical cord blood of women with allergy (four increased and three decreased in women with allergy), four miRNAs associated to addictive substance abuse status (two up- and two downregulated in women with addictive substance abuse) and eight miRNAs associated with maternal cigarette smoking during pregnancy.Conclusions. We successfully described differences in miRNA profiles in umbilical cord blood associated with basic characteristics connected with mother. Our data suggest that miRNAs in umbilical cord blood are detectable and associated with a wide range of maternal characteristics. These results indicate that miRNAs could potentially serve, and should be studied, as biomarkers for screening and diagnosis of pregnancy-associated complications and pathologies.