Structural and pharmacological characterization of the major brain nicotinic acetylcholine receptor subtype stably expressed in mouse fibroblasts.
Structural and pharmacological characterization of the major brain nicotinic acetylcholine receptor subtype stably expressed in mouse fibroblasts.
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发表时间：
1991-10
影响因子：
3.6
通讯作者：
Paul J. Whiting;R. Schoepfer;Jon Lindstrom;T. Priestley
中科院分区：
文献类型：
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作者：
Paul J. Whiting;R. Schoepfer;Jon Lindstrom;T. Priestley
在此之前，我们纯化了脑烟碱乙酰胆碱受体（AChR）的主要亚型，分析了它的结构，发现它由两种亚基组成，其cDNA编码的序列被称为α 4和β 2。在这里，我们表达这些cDNA从鸡脑中稳定转染成纤维细胞。我们通过合成证明，这些cDNA编码预期大小的亚基多肽，其共组装形成具有与天然乙酰胆碱受体相同大小的受体大分子。此外，我们表明，表达的乙酰胆碱受体表现出配体结合药理学的天然脑乙酰胆碱受体和乙酰胆碱门控离子通道的功能。
Previously, we purified the predominant subtype of brain nicotinic acetylcholine receptor (AChR), analyzed its structure, and found that it was composed of two kinds of subunit, with sequences encoded by cDNAs termed alpha 4 and beta 2. Here we express these cDNAs from chicken brain in stably transfected fibroblasts. We demonstrate by synthesis that these cDNAs encode subunit polypeptides of the expected sizes, which coassemble to form receptor macromolecules having the same size as native AChRs. Additionally, we demonstrate that the expressed AChRs exhibit the ligand-binding pharmacology of native brain AChRs and function as acetylcholine-gated ion channels.