Wnt and Shh signals regulate neural stem cell proliferation and differentiation in the optic tectum of adult zebrafish
Wnt and Shh signals regulate neural stem cell proliferation and differentiation in the optic tectum of adult zebrafish
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DOI：
10.1002/dneu.22509
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发表时间：
2017-10-01
影响因子：
3
通讯作者：
Ohshima, Toshio
中科院分区：
文献类型：
--
作者：
Shitasako, Shiori;Ito, Yoko;Ohshima, Toshio
成体神经发生在以斑马鱼为代表的硬骨鱼中比在哺乳动物中更常见。因此，斑马鱼被认为是研究成体神经发生的合适模型，其调控分子机制仍然知之甚少。我们的前期研究发现，神经上皮样神经干细胞（NSCs）位于背内侧区的边缘。我们还发现Notch信号抑制了该区域的NSC增殖。在本研究中，我们报告的表达Wnt和Shh信号成分在这一地区的视顶盖。此外，Wnt和Shh信号传导抑制剂抑制NSC增殖，表明这些途径促进NSC增殖。Shh对于维持Sox 2阳性NSC是特别需要的。我们的实验数据也表明这些信号通路参与神经干细胞的神经分化。(c)2017 Wiley Periodicals，Inc.开发神经生物学77：1206-1220，2017
Adult neurogenesis occurs more commonly in teleosts, represented by zebrafish, than in mammals. Zebrafish is therefore considered a suitable model to study adult neurogenesis, for which the regulatory molecular mechanisms remain little known. Our previous study revealed that neuroepithelial-like neural stem cells (NSCs) are located at the edge of the dorsomedial region. We also showed that Notch signaling inhibits NSC proliferation in this region. In the present study, we reported the expression of Wnt and Shh signaling components in this region of the optic tectum. Moreover, inhibitors of Wnt and Shh signaling suppressed NSC proliferation, suggesting that these pathways promote NSC proliferation. Shh is particularly required for maintaining Sox2-positive NSCs. Our experimental data also indicate the involvement of these signaling pathways in neural differentiation from NSCs. (c) 2017 Wiley Periodicals, Inc. Develop Neurobiol 77: 1206-1220, 2017