The involvement of the chemokine receptor CXCR2 in neutrophil recruitment in LPS-induced inflammation and in Mycobacterium avium infection
The involvement of the chemokine receptor CXCR2 in neutrophil recruitment in LPS-induced inflammation and in Mycobacterium avium infection
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DOI：
10.1046/j.1365-3083.2002.01097.x
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发表时间：
2002-06-01
影响因子：
3.7
通讯作者：
Appelberg, R
中科院分区：
文献类型：
--
作者：
Gonçalves, AS;Appelberg, R
CXC 受体 2 (CXCR2) 趋化因子受体基因敲除小鼠用于研究急性和慢性炎症反应期间中性粒细胞的募集。当腹膜内或气管内用脂多糖 (LPS) 治疗时，与对照小鼠相比，这些动物在前 24-48 小时内的中性粒细胞募集显着减少。在鸟分枝杆菌腹腔感染15天期间，与对照小鼠相比，基因敲除小鼠腹腔内的中性粒细胞数量显着减少。相比之下，在产气性鸟支原体感染期间，中性粒细胞向肺部的募集在整个 60 天的研究中并未受到 CXCR2 突变的影响。最后，我们没有发现突变对受感染动物的分枝杆菌生长有任何影响。这些发现表明，CXCR2 可能本质上参与了急性炎症反应，其中观察到中性粒细胞的早期和快速募集。
Knockout mice for CXC receptor 2 (CXCR2) chemokine receptor were used to study the recruitment of neutrophils during acute and chronic inflammatory responses. When treated with lipopolysaccharide (LPS), either intraperitoneally or intratracheally, these animals had a significant reduction in the neutrophil recruitment in the first 24-48 h as compared with control mice. During 15 days of intraperitoneal infection by Mycobacterium avium, the knockout mice showed significantly reduced numbers of neutrophils in the peritoneal cavity as compared with the control mice. In contrast, the recruitment of neutrophils to the lungs during an aerogenic M. avium infection was not affected by the CXCR2 mutation throughout the 60 days of the study. Finally, we could not find any impact of the mutation on the mycobacterial growth of the infected animals. These findings indicate that CXCR2 may be essentially involved in acute inflammatory responses where an early and rapid recruitment of neutrophils is observed.