The chick chorioallantoic membrane as a novel in vivo model for the testing of biomaterials
The chick chorioallantoic membrane as a novel in vivo model for the testing of biomaterials
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DOI：
10.1002/jbm.10152
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发表时间：
2002-11-01
期刊：
影响因子：
--
通讯作者：
Moussy, F
中科院分区：
文献类型：
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作者：
Valdes, TI;Kreutzer, D;Moussy, F
目前用于测试生物材料的体内模型是时间和劳动密集型以及昂贵的。本文介绍了一种新的方法来测试生物材料在体内使用的绒毛尿囊膜（CAM）的发展鸡胚，作为替代传统的哺乳动物模型。将受精的鸡蛋孵育4天，此时在蛋壳中切开一个小窗口。孵育1周后，CAM接受几种试验材料，包括内毒素LPS、棉线和硅橡胶管。1天和1周后，使用大体、组织学和扫描电子显微镜评价来评估对供试品的组织反应。对绒毛尿囊膜对生物材料的炎症反应进行了充分表征，发现其与哺乳动物反应相似，并且还发现其根据试验材料而变化。我们还发现，测试材料的结构和几何形状极大地影响了CAM中样品的掺入。CAM的组织反应与哺乳动物模型的相似性，加上低成本、简单性和通过外壳窗口连续可视化测试部位的可能性，使得该动物模型对于生物材料的快速体内筛选特别有吸引力。(C)2002 Wiley Periodicals，Inc.
Current in vivo models for testing biomaterials are time and labor intensive as well as expensive. This article describes a new approach for testing biomaterials in vivo using the chorioallantoic membrane (CAM) of the developing chicken embryo, as an alternative to the traditional mammalian models. Fertilized chicken eggs were incubated for 4 days, at which time a small window was cut in the shell of the egg. After 1 week of incubation, the CAM received several test materials, including the endotoxin LPS, a cotton thread and a Silastic tubing. One day and 1 week later, the tissue response to the test materials was assessed using gross, histological, and scanning electron microscope evaluations. The inflammatory response of the chorioallantoic membrane to biomaterials was fully characterized and found to be similar to that of the mammalian response and was also seen to vary according to test materials. We also found that the structure and geometry of the test materials greatly influenced the incorporation of the samples in the CAM. The similarity of the tissue response of the CAM with the mammalian models, plus the low cost, simplicity, and possibility to continuously visualize the test site through the shell window make this animal model particularly attractive for the rapid in vivo screening of biomaterials. (C) 2002 Wiley Periodicals, Inc.