Imaging electrical excitation inside the myocardial wall.
Imaging electrical excitation inside the myocardial wall.
复制标题
在心肌壁内进行成像电激发。
DOI：
10.1364/boe.2.000620
复制
发表时间：
2011-02-16
影响因子：
3.4
通讯作者：
Pertsov AM
中科院分区：
文献类型：
--
作者：
Mitrea BG;Caldwell BJ;Pertsov AM
心律失常通常是由起源于心肌壁深处的异位膜去极化引起的。在这里，我们提出了一种新的方法，利用一种新型的近红外电压敏感荧光染料DI-4-ANBDQBS来确定这种退偏振源的三维坐标。我们在猪左、右室心肌(厚度8-18 mm)和模拟心肌组织光学特性的模型上测试了该方法。该方法利用交替的透光方法，该方法包括比较在两个交替的照明方向上产生的同时记录的广域荧光和透光图像对。记录由两个面对心脏心内膜和心外膜表面的CCD摄像机以高达3 kHz的帧速率同时拍摄。在活体准备中，我们能够定位除极波的起源，在透壁方向上的精度为±1.3 mm，在图像平面上的精度为3 mm。检测的准确性与室壁内声源的深度无关。
Cardiac arrhythmias are often triggered by ectopic membrane depolarization originating deep inside the myocardial wall. Here we propose a new method utilizing a novel near-infrared voltage-sensitive fluorescent dye DI-4-ANBDQBS to determine the three-dimensional (3D) coordinates of the sources of such depolarization. We tested the method in live preparations of pig left and right ventricular myocardium (thickness 8-18 mm) and phantoms imitating the optical properties of myocardial tissue. The method utilizes an alternating transillumination approach that involves comparing pairs of simultaneously recorded broad-field epifluorescence and transillumination images produced at two alternating directions of illumination. Recordings were taken simultaneously by two CCD cameras facing the endocardial and epicardial surfaces of the heart at a frame rate up to 3 KHz. In live preparations, we were able to localize the origin of the depolarization wave with a precision of ±1.3mm in the transmural direction and 3 mm in the image plane. The accuracy of detection was independent of the depth of the source inside ventricular wall.