Rapid separation of amino acid phenylthiohydantoins by isocratic high-performance liquid chromatography.
Rapid separation of amino acid phenylthiohydantoins by isocratic high-performance liquid chromatography.
复制标题
采用等度高效液相色谱法快速分离氨基酸苯硫基乙内酰脲。
DOI：
10.1016/0003-2697(81)90223-2
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发表时间：
1981
影响因子：
2.9
通讯作者：
Tarr,GE
中科院分区：
文献类型：
--
作者：
Tarr,GE
采用基于缓冲乙腈水溶液、ODS固定相和运行过程中增加的程序化流速的等度方法，可在6至9 min内鉴别测序中衍生的所有常见苯硫代海因氨基酸。与恒定流量的分析需要16分钟。洗脱溶剂的百分比，离子强度，阴离子，阳离子，pH值和温度的变化的影响进行了定义。
All common phenylthiohydantoin amino acids derived from sequencing are identifiable in 6 to 9 min with an isocratic method based on buffered aqueous acetonitrile, an ODS stationary phase, and programmed flow which increases during the run. With constant flow the analysis requires 16 min. The effects of variation in percentage eluting solvent, ionic strength, anion, cation, pH, and temperature are defined.