Protective effects of coenzyme Q10 and α-tocopherol against free radical-mediated liver cell injury.
Protective effects of coenzyme Q10 and α-tocopherol against free radical-mediated liver cell injury.
复制标题
辅酶 Q10 和 α-生育酚对自由基介导的肝细胞损伤的保护作用。
作者：
T. Matsura;K. Yamada;T. Kawasaki
为了进一步证实还原型辅酶 Q 同系物 (CoQnH2) 和 α-生育酚 (α-Toc) 的抗氧化作用，我们将分离的大鼠肝细胞与水溶性自由基引发剂 2,2'-偶氮二(2-脒基丙烷)二盐酸盐 (AAPH) 在存在或不存在外源添加的辅酶 Q10 (CoQ10) 或 α-Toc 的情况下孵育，在 95% 氧气和 5% 二氧化碳的气氛下，37°C 3 小时。在未添加AAPH的对照实验中，证实添加的CoQ10和α-Toc被掺入细胞中，并且一些CoQ10转化为CoQ10H2。肝细胞与 50 mM AAPH 一起孵育会导致硫代巴比妥酸反应物质的形成和细胞活力的降低，并且两者均受到外源添加的 CoQ10 或 α-Toc 的抑制，并呈剂量依赖性。通过添加 AAPH，观察到内源性 CoQ9H2 和 α-Toc 水平的降低。添加 CoQ10 剂量依赖性地抑制 CoQ9H2 氧化为 CoQ9，而添加 α-Toc 则不然。这些数据表明 CoQnH2 和 α-Toc 都充当抗氧化剂，并且可以抑制自由基介导的细胞损伤。
In an attempt to provide further confirmation of the antioxidant role of reduced form of coenzyme Q homologue (CoQnH2) and α-tocopherol (α-Toc), we incubated isolated rat hepatocytes with a water-soluble radical initiator, 2,2'-azobis(2-amidinopropane)dihydrochloride (AAPH) in the presence or absence of exogenously added coenzyme Q10 (CoQ10) or α-Toc for 3 h at 37°C under an atmosphere of 95% oxygen and 5% carbon dioxide. In the control experiment without adding AAPH it was confirmed that added CoQ10 and α-Toc were incorporated into the cells and some CoQ10 were converted to CoQ10H2. Incubation of hepatocytes with 50 mM AAPH resulted in the formation of thiobarbituric acid-reactive substances and the decrease in cell viability and both were inhibited by exogenously added CoQ10 or α-Toc in a dose-dependent manner. The decrease in endogenous CoQ9H2 and α-Toc levels was observed by the addition of AAPH. Addition of CoQ10 inhibited the oxidation of CoQ9H2 to CoQ9 dose-dependently while the addition of α-Toc did not. These data suggest that both CoQnH2 and α-Toc act as antioxidants and can inhibit free radical-mediated cell injury.
DOI：
10.1073/pnas.87.12.4879
发表时间：
1990-06-01
影响因子：
11.1
作者：
FREI, B;KIM, MC;AMES, BN
通讯作者：
AMES, BN
影响因子：
7.4
作者：
KAGAN, VE;SERBINOVA, EA;PACKER, L
通讯作者：
PACKER, L