Cell Viability and Tissue Response of High Molecule Weight DNA/protamine Complex
Cell Viability and Tissue Response of High Molecule Weight DNA/protamine Complex
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高分子量 DNA/鱼精蛋白复合物的细胞活力和组织反应
DOI：
10.11223/jarde.8.188
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发表时间：
2011
期刊：
影响因子：
--
通讯作者：
T.
中科院分区：
文献类型：
--
作者：
Mori;N.;Iwahashi;T.;Ohno;J.;Shinozaki;Y.;Sakagami;R.;Mitarai;M.;Fukushima;T.
通过高分子量DNA与鱼精蛋白反应制备了DNA/鱼精蛋白复合物。所得高分子量DNA/鱼精蛋白复合物为水不溶性白色粉末。产率为75%。DNA与鱼精蛋白的摩尔结合比（鱼精蛋白的摩尔/DNA的1个碱基对）为0.129。SEM观察表明，所制备的复合物具有多孔结构。多孔盘的制备方法简单。在使用MC 3 T3-E1小鼠成骨细胞的细胞培养试验中，DNA/鱼精蛋白复合物的细胞活力为87.1%至99.0%。体内实验表明，复合物植入大鼠背部皮肤后，表现出轻微的组织反应。复合物在植入过程中逐渐降解，植入后30天仍有部分复合物滞留。这些结果表明，高分子量DNA不影响DNA/鱼精蛋白复合物的细胞毒性和组织反应。
The DNA/protamine complex was prepared from the reaction of high molecule weight DNA and protamine. The obtained high molecule weight DNA/protamine complex was water-insoluble white powder. The yield was 75%. The molar binding ratio (molar of protamine/1 base pair of DNA) DNA to protamine in was 0.129. SEM observation revealed that prepared complex had a porous structure. The porous disk could be easily prepared. In cell culture tests using MC 3T3-E1 mouse osteoblast cells, cell viabilities for the DNA/protamine complex were from 87.1% to 99.0%. In vivo test, complex showed a mild tissue response after the implantation into the back of skin of rats. The complex was gradually biodegraded during implantation, and a part of complex resided 30 days after implantation. These results suggested that high molecule weight DNA do not influence cytotoxicity and tissue response of DNA/protamine complex.