Atomic-Level Passivation of Individual Upconversion Nanocrystal for Single Particle Microscopic Imaging
Atomic-Level Passivation of Individual Upconversion Nanocrystal for Single Particle Microscopic Imaging
复制标题
用于单粒子显微成像的单个上转换纳米晶体的原子级钝化
DOI：
10.1002/adfm.201906137
复制
发表时间：
2020
影响因子：
19
通讯作者：
Xu Xuhui
中科院分区：
文献类型：
--
作者：
Min Qiuhong;Lei Jing;Guo Xin;Wang Ting;Yang Qihua;Zhou Dacheng;Yu Xue;Yu Siu Fung;Qiu Jianbei;Zhan Qiuqiang;Xu Xuhui
镧系元素掺杂的上转换纳米粒子（UCNPs）在单分子探针和高分辨率显示方面有着重要的应用。然而，它们的主要障碍之一是弱发光，这在单粒子水平上仍然是一个巨大的挑战。在这里，由于Yb 3+介导的局部光热效应，LuF 3：Yb 3+，Er 3+菱形片状UCNP实现了484倍的发光增强，并且它们的原始形态，尺寸和良好的结晶性得到了很好的保留。这些数据表明，UCNPs的表面原子结构以及从非晶到有序晶体结构的转移是通过利用由Yb 3+离子对980 nm光的定向吸收产生的局部光热转换来调制的。通过超分辨率受激发射耗尽获得的共焦发光图像也显示了单个LuF 3：Yb 3+，Er 3+纳米颗粒的极大增强;高信噪比图像表明激光治疗技术为单颗粒成像和实际应用打开了大门。
Lanthanide‐doped upconversion nanoparticles (UCNPs) have significant applications for single‐molecule probes and high‐resolution display. However, one of their major hurdles is the weak luminescence, and this remains a grand challenge to achieve at the single‐particle level. Here, 484‐fold luminescence enhancement in LuF3:Yb3+, Er3+rhombic flake UCNPs is achieved, thanks to the Yb3+‐mediated local photothermal effect, and their original morphology, size, and good dispersibility are well preserved. These data show that the surface atomic structure of UCNPs as well as transfer from amorphous to ordered crystal structure is modulated by making use of the local photothermal conversion that is generated by the directional absorption of 980 nm light by Yb3+ions. The confocal luminescence images obtained by super‐resolution stimulated emission depletion also show the great enhancement of individual LuF3:Yb3+, Er3+nanoparticles; the high signal‐to‐noise ratio images indicate that the laser treatment technology opens the door for single particle imaging and practical application.