Nanoporous surfaces as harvesting agents for mass spectrometric analysis of peptides in human plasma
Nanoporous surfaces as harvesting agents for mass spectrometric analysis of peptides in human plasma
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DOI：
10.1021/pr050417
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发表时间：
2006-05-01
影响因子：
4.4
通讯作者：
Ferrari, M
中科院分区：
文献类型：
--
作者：
Gaspari, M;Cheng, MMC;Ferrari, M
已经开发了基于二氧化硅的纳米多孔表面，以从人血浆中捕获低分子量肽。通过使用基质辅助激光解吸/电离飞行时间质谱（MALDI-TOF MS）作为检测和评估结合分子的手段，对收获的肽进行质谱分析。生成了由800-10 000 m/z范围内的约70个峰组成的肽图谱。该方法可以允许检测ng/mL浓度水平的小肽，无论是在标准溶液或血浆中。对于与硅基纳米多孔表面孵育的标准蛋白质和肽的混合物，观察到相同的分子截止效应。
Silica-based nanoporous surfaces have been developed in order to capture low molecular weight peptides from human plasma. Harvested peptides were subjected to mass spectrometric analysis by using matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) as a means of detecting and assessing the bound molecules. Peptide profiles consisting of about 70 peaks in the range 800-10 000 m/z were generated. The method could allow detection of small peptides at ng/mL concentration levels, either in standard solutions or in plasma. The same molecular cutoff effect was observed for mixtures of standard proteins and peptides incubated with silicon-based nanoporous surfaces.