Can hepatocytes proliferate when transplanted into the spleen? Demonstration by autohistoradiography in the rat.
Can hepatocytes proliferate when transplanted into the spleen? Demonstration by autohistoradiography in the rat.
复制标题
肝细胞移植到脾脏后可以增殖吗？
DOI：
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发表时间：
1987
期刊：
影响因子：
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通讯作者：
Recaredo Infante
中科院分区：
文献类型：
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作者：
Bernard Nordlinger;S. R. Wang;M. Bouma;N. Verthier;K. Hillan;R. Delelo;Recaredo Infante
本研究的目的是研究肝细胞移植到脾脏后的增殖，以便将这项技术应用于慢性肝病的治疗。将Wistar Furth大鼠原位胶原酶灌注技术分离的肝细胞注射到三组同系大鼠的脾实质中：正常肝脏对照组（第1组）、75%肝切除组（第2组）和端侧门腔静脉分流组（第3组）。在肝细胞移植后第1、3、7和15天，腹腔注射0.6 microCi/g体重的[3 H]-胸苷后，通过放射自显影术研究移植肝细胞的增殖。在研究期间，主要在第2组和第3组中观察到移植肝细胞显著掺入[3 H]-胸苷。虽然延迟，但在门腔静脉分流动物中，该结合持续且幅度最大。移植的肝细胞在脾脏中增殖的能力，特别是在门腔静脉分流后，表明这种方法可能在慢性肝病的治疗中具有治疗应用。
The aim of this work was to study hepatocyte multiplication after transplantation into the spleen, in order to apply this technique to the treatment of chronic liver disease. Hepatocytes isolated by an in situ collagenase perfusion technique in Wistar Furth rats were injected into the splenic parenchyma of three groups of syngeneic rats: controls with normal liver (group 1), 75% hepatectomies (group 2), and end-to-side portacaval shunts (group 3). The proliferation of transplanted hepatocytes was studied by autohistoradiography after the intraperitoneal administration of 0.6 microCi/g body weight of [3H]-thymidine, at 1, 3, 7 and 15 days after transplantation of hepatocytes. Significant incorporation of [3H]-thymidine by the transplanted hepatocytes during the study period was observed mostly in groups 2 and 3. The incorporation, although delayed was sustained and of greatest magnitude in the portacaval-shunted animals. The ability of transplanted hepatocytes to proliferate in the spleen, particularly after a portacaval shunt, indicates that this procedure may have therapeutic applications in the treatment of chronic liver disease.