Combining Ability for Phytophthora infestans Quantitative Resistance from Wild Tomato
Combining Ability for Phytophthora infestans Quantitative Resistance from Wild Tomato
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DOI：
10.2135/cropsci2014.04.0286
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发表时间：
2015
期刊：
影响因子：
2.3
通讯作者：
J. E. Haggard;D. A. St.Clair
中科院分区：
文献类型：
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作者：
J. E. Haggard;D. A. St.Clair
将赋予晚疫病定量抗性的等位基因[Phytophthora infestans (Mont.) de Bary]从抗性野生番茄(Solanum habrochaites Knapp and Spooner)基因渗入到栽培番茄[Solanum lycopersicum (L.)]中。对两组亚近等基因系（sub-NIL）进行标记选择，使其各自包含来自 S. habrochaites 5 号或 11 号染色体的抗性 QTL 等位基因。我们对亚 NIL 进行了配合力研究，以评估抗性 QTL 等位基因在育种 F₁ 杂交品种中的潜在效用，并鉴定具有最高配合力的晚疫病抗性亚 NIL。 sub-NIL 与阶乘交配设计（设计 II）中的近交测试者交配。来自这两组亚NIL的F 1 杂种后代，其中每个杂种对于第5或11号染色体上的一个或多个QTL抗性等位基因是杂合的，在重复的田间和生长室实验中评估了致病疫霉的抗性。在生长室中发现了配合力的差异，但在田间实验中没有发现，这表明亚NIL对其田间的F₁杂种后代具有相似水平的抗性，但在生长室中的水平不同。在田间和生长室实验中检测到显着的 QTL × 环境相互作用。我们鉴定了在 F₁ 杂交组合中表达致病疫霉抗性的亚 NIL，这表明这些亚 NIL 可用于培育具有增强抗性的 F₁ 杂交品种。来自每个亚NIL亲本的F 1 杂种中对致病疫霉的定量抗性表达是由来自S.habrochaites的多个QTL等位基因提供的。
Alleles conferring quantitative resistance to late blight disease [Phytophthora infestans (Mont.) de Bary] were introgressed into cultivated tomato [Solanum lycopersicum (L.)] from resistant wild tomato (Solanum habrochaites Knapp and Spooner). Two sets of sub-near-isogenic lines (sub-NILs) were marker-selected so that each contained resistance QTL alleles from S. habrochaites chromosome 5 or 11. We conducted a combining ability study with the sub-NILs to evaluate the potential utility of the resistance QTL alleles for breeding F₁ hybrid cultivars and identify sub-NILs with the highest combining ability for late blight disease resistance. The sub-NILs were mated with inbred testers in factorial mating designs (Design II). F₁ hybrid progeny from these two sets of sub-NILs, in which each hybrid was heterozygous for one or more QTL resistance alleles on chromosome 5 or 11, were evaluated for P. infestans resistance in replicated field and growth chamber experiments. Differences in combining ability were found in growth chamber but not field experiments, suggesting that sub-NILs contributed similar levels of resistance to their F₁ hybrid progeny in the field, but different levels in the growth chamber. Significant QTL × environment interactions were detected across field and growth chamber experiments. We identified sub-NILs that expressed P. infestans resistance in F₁ hybrid combinations, suggesting that these sub-NILs could be useful for breeding F₁ hybrid varieties with enhanced resistance. The expression of quantitative resistance to P. infestans in F₁ hybrids derived from each sub-NIL parent was provided by multiple QTL alleles from S. habrochaites.