Associations between Notch-2, Akt-1 and HER2/neu expression in invasive human breast cancer: A tissue microarray immunophenotypic analysis on 98 patients
Associations between Notch-2, Akt-1 and HER2/neu expression in invasive human breast cancer: A tissue microarray immunophenotypic analysis on 98 patients
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DOI：
10.1159/000110024
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发表时间：
2007-01-01
期刊：
影响因子：
5
通讯作者：
Franco, Vito
中科院分区：
文献类型：
--
作者：
Florena, Ada Maria;Tripodo, Claudio;Franco, Vito
目的：我们的目的是研究乳腺癌的生物学和表型特征与肿瘤进展和预后之间的关系。研究方法：采用组织芯片技术检测98例浸润性乳腺癌雌激素和孕激素受体Ki-67、HER 2、Akt-1和Notch-2的免疫组化表达。收集每例患者的肿瘤组织型、组织学分级、肿瘤大小和淋巴结状态的数据并纳入分析中。结果：从我们的数据分析中得出了组织学和/或免疫表型特征之间的几个显著相关性。在雌激素和孕激素受体、肿瘤分级和增殖指数、肿瘤分级和HER 2、Akt-1和雌激素受体以及Notch-2和HER 2之间观察到正相关。激素受体与肿瘤分级、激素受体与HER 2、Akt-1与肿瘤分级、Akt-1与淋巴结浸润呈负相关。结论：我们的研究结果显示，存在许多雌激素受体阳性肿瘤，Akt-1表达，分化程度更好，无淋巴结受累，沿着HER 2阳性肿瘤，Notch-2表达强烈，支持Notch和Akt在乳腺癌进展中的作用，并表明它们也可能代表新的有吸引力的治疗靶点。版权所有（c）2007 S. Karger AG，巴塞尔。
Objective: We aimed to investigate the existence of associations between well- established and newly recognized biological and phenotypic features of breast cancer involved in tumor progression and prognosis. Methods: Ninety- eight cases of invasive breast cancer were assessed for the immunohistochemical expression of estrogen and progesterone receptors, Ki-67, HER2, Akt-1, and Notch-2, using the tissue microarray technique. Data regarding tumor histotype, histological grade, tumor size and lymph node status were collected for each patient and included in the analysis. Results: Several significant associations between histological and/ or immunophenotypic features came from the analysis of our data. Positive associations were observed between estrogen and progesterone receptors, tumor grade and proliferation index, tumor grade and HER2, Akt-1 and estrogen receptors, and Notch-2 and HER2. Inverse associations were noted between hormone receptors and tumor grade, hormone receptors and HER2, Akt-1 and tumor grade, and Akt-1 and nodal invasion. Conclusions: Our results, showing the existence of a number of estrogen receptor-positive tumors with Akt-1 expression, better degree of differentiation, and no lymph node involvement, along with the presence of HER2-positive tumors with strong Notch-2 expression, support the role of Notch and Akt in breast cancer progression and suggest that they may also represent new appealing therapeutic targets. Copyright (c) 2007 S. Karger AG, Basel.