Variable region sequences of murine IgM anti-IgG monoclonal autoantibodies (rheumatoid factors). II. Comparison of hybridomas derived by lipopolysaccharide stimulation and secondary protein immunization.
Variable region sequences of murine IgM anti-IgG monoclonal autoantibodies (rheumatoid factors). II. Comparison of hybridomas derived by lipopolysaccharide stimulation and secondary protein immunization.
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鼠 IgM 抗 IgG 单克隆自身抗体（类风湿因子）的可变区序列。
DOI：
10.1084/jem.165.4.970
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发表时间：
1987
期刊：
影响因子：
--
通讯作者：
Weigert,M
中科院分区：
文献类型：
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作者：
Shlomchik,M;Nemazee,D;vanSnick,J;Weigert,M
我们已经获得了完整的可变区mRNA序列的11 LPS衍生的和14二次免疫衍生的单克隆IgM抗IgG抗体（类风湿因子，RF）。这些序列的比较分析表明，多克隆活化后衍生的单克隆RF在结构上非常类似于二次蛋白免疫后衍生的RF。进行本研究以评价两种先前描述的现象之间的潜在关系：（a）在对蛋白抗原的二次应答期间，RF以等于或超过免疫原特异性抗体的量产生;和（B）多克隆活化后产生RF的B细胞的频率相当高; 3- 10%。目前还不清楚是否LPS刺激的细胞产生IgM抗IgG，在体外试验中检测到的细胞与体内刺激后产生RF的细胞。然而，在两种RF中发现的抗原受体的相似性表明，在LPS刺激后检测到的大多数或所有产生RF的B细胞也将在二次免疫应答期间被刺激。因此，在非特异性刺激后，相对大量的B细胞可以产生RF，这为伴随二次免疫应答的RF产生的幅度提供了解释。
We have obtained the complete variable region mRNA sequences of 11 LPS-derived and 14 secondary immunization-derived monoclonal IgM anti-IgG antibodies (rheumatoid factors, RFs). A comparative analysis of these sequences showed that monoclonal RFs derived after polyclonal activation are structurally very similar to RFs derived after secondary protein immunization. This study was undertaken to evaluate the potential relationship between two previously described phenomena: (a) during a secondary response to a protein antigen, RF is produced in quantities that equal or exceed the immunogen-specific antibody; and (b) the frequency of B cells that make RF after polyclonal activation is quite high; 3-10%. It has been unclear whether LPS-stimulated cells that produce IgM anti-IgG that is detected by an in vitro assay are related to the cells that produce RF after in vivo stimulation. The similarity of the antigen receptors found in the two types of RF, however, suggests that most or all of the RF-producing B cells detected after LPS stimulation would also be stimulated during the secondary immune response. Thus, the presence of relatively large number of B cells that can make RF after nonspecific stimulation provides an explanation for the magnitude of RF production accompanying the secondary immune response.