Structure of the first PDZ domain of human PSD-93
Structure of the first PDZ domain of human PSD-93
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DOI：
10.1107/s1744309109043267
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发表时间：
2009-12-01
影响因子：
0.9
通讯作者：
Gajhede, Michael
中科院分区：
文献类型：
--
作者：
Fiorentini, Monica;Nielsen, Ann Kallehauge;Gajhede, Michael
人PSD-93的PDZ1结构域的晶体结构已被确定为2.0埃分辨率。PDZ1结构域形成一种晶体三聚体，预计该三聚体在溶液中也是稳定的。对三聚体稳定的主要贡献似乎来自涉及PDZ1末端的PDZ1-PDZ2连接区的相互作用，这意味着在全长PSD-93中观察到的寡聚不具有生物学意义。比较PSD-93PDZ1与已报道的PSD-93PDZ2和PDZ3的结构以及与PSD-95PDZ1密切相关的人PSD-95PDZ1的结构，发现它们在氨基酸组成上非常相似。然而，在PSD-95中，裂隙明显缩小。这可能是PSD-93PDZ1和PSD-95PDZ1多肽选择性的部分基础。
The crystal structure of the PDZ1 domain of human PSD-93 has been determined to 2.0 angstrom resolution. The PDZ1 domain forms a crystallographic trimer that is also predicted to be stable in solution. The main contributions to the stabilization of the trimer seem to arise from interactions involving the PDZ1-PDZ2 linker region at the extreme C-terminus of PDZ1, implying that the oligomerization that is observed is not of biological significance in full-length PSD-93. Comparison of the structures of the binding cleft of PSD-93 PDZ1 with the previously reported structures of PSD-93 PDZ2 and PDZ3 as well as of the closely related human PSD-95 PDZ1 shows that they are very similar in terms of amino-acid composition. However, the cleft is significantly narrower in PSD-95. This could be part of the basis of peptide selectivity between PSD-93 PDZ1 and PSD-95 PDZ1.