Male C57BL6/N and C57BL6/J Mice Respond Differently to Constant Light and Running-Wheel Access
Male C57BL6/N and C57BL6/J Mice Respond Differently to Constant Light and Running-Wheel Access
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DOI：
10.3389/fnbeh.2019.00268
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发表时间：
2019-12-10
影响因子：
3
通讯作者：
Seggio, Joseph A.
中科院分区：
文献类型：
--
作者：
Capri, Kimberly M.;Maroni, Marissa J.;Seggio, Joseph A.
此前的研究表明，暴露在昼夜节律紊乱中会对整体健康和行为产生负面影响。最近的研究表明，即使菌株具有相似的遗传背景，它们对昼夜节律中断的行为和生理反应也存在差异。因此，我们研究了带转轮通道的恒定室内光线(LL)对来自Jackson实验室的雄性C57BL6/J和来自Charles River实验室的C57BL6/N小鼠的行为和生理的影响。小鼠被暴露在12：12的明暗(LD)循环或LL中，并被给予标准的家庭笼子或带转轮的笼子。在LD或LL饲养6周后，观察它们对行为分析(开阔场地、明暗箱、新奇物体)的反应和代谢指标。在标准LD下，与C57BL6/N小鼠相比，C57BL6/J小鼠表现出更多的运动活动和更少的探索行为。在LL中，C57BL6/J小鼠出现更大的周期延长和焦虑增加，而C57BL6/N小鼠体重增加，探索行为没有变化。C57BL6/J小鼠也减少了通过转轮进入的探索，而C57BL6/N小鼠没有。这些结果进一步表明，C57BL/6亚株对昼夜节律干扰和车轮运行进入表现出不同的行为和生理反应。
Previous studies have shown that exposure to circadian disruption produces negative effects on overall health and behavior. More recent studies illustrate that strain differences in the behavioral and physiological responses to circadian disruption exist, even if the strains have similar genetic backgrounds. As such, we investigated the effects of constant room-level light (LL) with running-wheel access on the behavior and physiology of male C57BL6/J from Jackson Laboratories and C57BL6/N from Charles River Laboratories mice. Mice were exposed to either a 12:12 light-dark (LD) cycle or LL and given either a standard home cage or a cage with a running-wheel. Following 6 weeks of LD or LL, their response to behavioral assays (open-field, light-dark box, novel object) and measures of metabolism were observed. Under standard LD, C57BL6/J mice exhibited increased locomotor activity and reduced exploratory behavior compared to C57BL6/N mice. In LL, C57BL6/J mice had greater period lengthening and increased anxiety, while C57BL6/N mice exhibited increased weight gain and no change in exploratory behavior. C57BL6/J mice also decreased exploration with running-wheel access while C57BL6/N mice did not. These results further demonstrate that C57BL/6 substrains exhibit different behavioral and physiological responses to circadian disruption and wheel-running access.