The mitogen-activated protein kinases WIPK and SIPK regulate the levels of jasmonic and salicylic acids in wounded tobacco plants
The mitogen-activated protein kinases WIPK and SIPK regulate the levels of jasmonic and salicylic acids in wounded tobacco plants
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DOI：
10.1111/j.1365-313x.2006.03003.x
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发表时间：
2007-03-01
期刊：
影响因子：
7.2
通讯作者：
Ohashi, Yuko
中科院分区：
文献类型：
--
作者：
Seo, Shigemi;Katou, Shinpei;Ohashi, Yuko
在烟草（Nicotiana tabacum）中，受伤会导致两种丝裂原激活蛋白激酶（MAPK）、伤口诱导蛋白激酶（WIPK）和水杨酸（SA）诱导蛋白激酶（SIPK）快速激活，以及随后茉莉酸（JA）的积累。我们之前的研究表明，WIPK 的激活是伤口诱导 JA 的产生所必需的。然而，WIPK 的确切作用仍未解决。我们培育了转基因烟草植物，其中使用 RNA 干扰使 WIPK 或 SIPK 沉默，以确定 WIPK 和 SIPK 在伤口反应中的作用。此外，还产生了转基因烟草植物，其中 WIPK 和 SIPK 均被沉默，以检查它们具有冗余作用的可能性。在所有 WIPK、SIPK 和 WIPK/SIPK 沉默植物中，与非沉默植物相比，伤口诱导的 JA 产量减少。过度表达 NtMKP1（一种编码烟草 MAPK 磷酸酶的基因，可使 WIPK 和 SIPK 失活）的转基因植物在受伤时也表现出 JA 产量减少。在 WIPK/SIPK 沉默和 NtMKP1 过表达植物中，损伤导致 SA 和 SA 响应基因转录物的异常积累。这些结果表明 WIPK 和 SIPK 在响应伤害的 JA 产生中发挥重要作用，并且它们协同作用来控制 SA 生物合成。
In tobacco (Nicotiana tabacum), wounding causes rapid activation of two mitogen-activated protein kinases (MAPKs), wound-induced protein kinase (WIPK) and salicylic acid (SA)-induced protein kinase (SIPK), and the subsequent accumulation of jasmonic acid (JA). Our previous studies suggested that activation of WIPK is required for the production of wound-induced JA. However, the exact role of WIPK remains unresolved. We generated transgenic tobacco plants in which either WIPK or SIPK were silenced using RNA interference to define the roles of WIPK and SIPK in the wound response. In addition, transgenic tobacco plants were generated in which both WIPK and SIPK were silenced to examine the possibility that they have redundant roles. Wound-induced JA production was reduced compared with non-silenced plants in all of the WIPK-, SIPK- and WIPK/SIPK-silenced plants. Transgenic plants over-expressing NtMKP1, a gene encoding tobacco MAPK phosphatase, which inactivates WIPK and SIPK, also exhibited reduced JA production in response to wounding. In both WIPK/SIPK-silenced and NtMKP1-over-expressing plants, wounding resulted in an abnormal accumulation of both SA and transcripts for SA-responsive genes. These results suggest that WIPK and SIPK play an important role in JA production in response to wounding, and that they function cooperatively to control SA biosynthesis.