Colony-Stimulating Factor 2 (CSF-2) Improves Development and Posttransfer Survival of Bovine Embryos Produced in Vitro
Colony-Stimulating Factor 2 (CSF-2) Improves Development and Posttransfer Survival of Bovine Embryos Produced in Vitro
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DOI：
10.1210/en.2009-0481
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发表时间：
2009-11-01
期刊：
影响因子：
4.8
通讯作者：
Hansen, Peter J.
中科院分区：
文献类型：
--
作者：
Loureiro, Barbara;Bonilla, Luciano;Hansen, Peter J.
在这项研究中，我们测试了集落刺激因子2 （CSF2）作为一种调节分子，在着床前介导母体对胚胎发育的影响。我们的目的是验证CSF2对囊胚产量的影响，确定移植后的存活率，并评估CSF2处理后形成的囊胚的特性。在体外，CSF2增加了卵母细胞成为桑葚胚和囊胚的百分比。经CSF2处理的囊胚内细胞团细胞数量较多，内细胞团细胞与滋养外胚层细胞的比例较高。CSF2对细胞凋亡发生率无影响。从妊娠第30-35天妊娠率的提高可以看出，在受精后第5 - 7天用CSF2治疗可以提高胚胎存活率。此外，在受精后1-7天或5-7天使用CSF2治疗可减少30-35天后的妊娠损失。结果表明，CSF2可以影响胚胎发育，提高胚胎移植后存活能力。在如此狭窄的发育窗口期使用CSF2治疗会改变妊娠后期的胚胎功能，这一事实表明CSF2可能对发育中的胚胎施加表观遗传效应，导致胚胎和胎儿发育期间功能的持续变化。（内分泌学150:5046-5054,2009）
In this study, we tested the role of colony-stimulating factor 2 (CSF2) as one of the regulatory molecules that mediate maternal effects on embryonic development during the preimplantation period. Our objective was to verify effects of CSF2 on blastocyst yield, determine posttransfer survival, and evaluate properties of the blastocyst formed after CSF2 treatment. In vitro, CSF2 increased the percentage of oocytes that became morulae and blastocysts. Blastocysts that were treated with CSF2 tended to have a greater number of inner cell mass cells and had a higher ratio of inner cell mass to trophectoderm cells. There was no effect of CSF2 on the incidence of apoptosis. Treatment with CSF2 from d 5 to 7 after insemination increased embryonic survival as indicated by improved pregnancy rate at d 30-35 of gestation. Moreover, treatment with CSF2 from either d 1-7 or 5-7 after insemination reduced pregnancy loss after d 30-35. Results indicate that treatment with CSF2 can affect embryonic development and enhance embryo competence for posttransfer survival. The fact that treatment with CSF2 during such a narrow window of development altered embryonic function much later in pregnancy suggests that CSF2 may exert epigenetic effects on the developing embryo that result in persistent changes in function during the embryonic and fetal periods of development. (Endocrinology 150: 5046-5054, 2009)